Salidroside suppresses the multiple oncogenic activates and immune escape of lung adenocarcinoma through the circ_0009624‐mediated PD‐L1 pathway

• Published in: Thoracic cancer Vol. 14; no. 24; pp. 2493 - 2503
• Main Authors: Jin, Guilin, Ma, Mi, Yang, Chunyan, Zhen, Luo, Feng, Mingke
• Format: Journal Article
• Language: English
• Published: Melbourne John Wiley & Sons Australia, Ltd 01.08.2023; John Wiley & Sons, Inc; Wiley
• Subjects: Cancer therapies; Cell growth; Chinese medicine; Circ_0009624; Cytotoxicity; LUAD; Lung cancer; Lymphocytes; Medical research; Original; PD‐L1; Polymerase chain reaction; Procedure manuals; salidroside; Tumors; PD-L1; salidroside; Circ_0009624; LUAD
• ISSN: 1759-7706; 1759-7714
• DOI: 10.1111/1759-7714.15034

Background Lung adenocarcinoma (LUAD) is a fatal malignancy all over the world. Salidroside (SAL) is an active component extracted from Rhodiola rosea that has been reported to exert antitumor activity against several human cancers, containing lung adenocarcinoma (LUAD). The purpose of this study was to explore the effect and underlying mechanism of SAL in LUAD. Methods Cell viability, proliferation, migration, and invasion were measured using cell counting kit‐8 (CCK‐8), 5‐ethynyl‐2′‐deoxyuridine (EdU), and transwell assays. Effects of LUAD cells on the cytotoxicity, percentage, and death of CD8+ cells were detected using lactate dehydrogenase (LDH) and flow cytometry assays. Programmed cell death ligand 1 (PD‐L1) protein level was examined using western blot. Circ_0009624, enolase 1 (ENO1), and PD‐L1 levels were determined using real‐time quantitative polymerase chain reaction (RT‐qPCR). The biological role of SAL on LUAD tumor growth was assessed using the xenograft tumor model in vivo. Results SAL restrained LUAD cell proliferation, migration, invasion, and immune escape in vitro via modulating PD‐L1. Circ_0009624 expression was increased in LUAD. Applying SAL repressed circ_0009624 and PD‐L1 expression in LUAD cells. SAL treatment hindered suppressed various oncogenic activates and immune escape of LUAD cells by regulating the circ_0009624/PD‐L1 pathway. SAL blocked LUAD xenograft growth in vivo. Conclusion Applying SAL might constrain malignant phenotypes and immune escape of LUAD cells partially through the circ_0009624‐mediated PD‐L1 pathway, providing a novel insight for LUAD treatment. Circ_0009624 expression was increased in LUAD tissues and cells, and decreased in SAL‐treated LUAD cells. Furthermore, applying SAL might constrain malignant phenotypes and immune escape of LUAD cells partially through the circ_0009624‐mediated PD‐L1 pathway.