Uptake and Retention of Nanoplastics in Quagga Mussels

• Published in: Global challenges Vol. 4; no. 6; pp. 1800104 - n/a
• Main Authors: Merzel, Rachel L., Purser, Lauren, Soucy, Taylor L., Olszewski, Monica, Colón‐Bernal, Isabel, Duhaime, Melissa, Elgin, Ashley K., Banaszak Holl, Mark M.
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.06.2020; John Wiley and Sons Inc; Wiley
• Subjects: AFM‐IR; Atomic force microscopy; Beads; Carboxylic acids; Circulatory system; Experiments; Fluorescence; Fluorescence microscopy; Fluorescence spectroscopy; Food; Fourier transforms; Gills; Grooves; Infrared spectroscopy; microplastics; Microscopy; Mollusks; Mussels; nanoplastics; Nonnative species; Physiology; Plastics; Polystyrene; Polystyrene resins; PT‐IR; Siphons; Spectrum analysis; Studies; PT‐IR; AFM‐IR; microplastics; nanoplastics; mussels
• ISSN: 2056-6646; 2056-6646
• DOI: 10.1002/gch2.201800104

Here, a set of experiments to assess the feasibility of using an invasive and widespread freshwater mussel (Dreissena rostrformis bugensis) as a sentinel species for nanoplastic detection is reported. Under laboratory experimental conditions, mussels ingest and retain fluorescent polystyrene (PS) beads with carboxylic acid (COOH) termination over a size range of 200–2000 nm. The number of beads the mussels ingested is quantified using fluorescence spectroscopy and the location of the beads in the mussels is imaged using fluorescence microscopy. PS beads of similar size (1000–2000 nm) to mussels' preferred food are trafficked in the ciliated food grooves of the gills. Beads of all sizes are observed in the mussels' digestive tracts, indicating that the mussels do not efficiently reject the beads as unwanted foreign material, regardless of size. Fluorescence microscopy shows all sizes of beads are concentrated in the siphons and are retained there for longer than one month postexposure. Combined atomic force microscopy–infrared spectroscopy and photothermal infrared spectroscopy are used to locate, image, and chemically identify the beads in the mussel siphons. In sum, these experiments demonstrate the potential for using mussels, specifically their siphons, to monitor environmental accumulation of aquatic nanoplastics. Can quagga mussels (Dreissena rostriformis bugensis), a widespread and invasive freshwater species that alters local ecosystems, act as a sentinel species for detecting nanoplastics? In the laboratory, mussels ingest and retain 200–2000 nm fluorescent polystyrene beads, which are in the size range for the mussels' preferred food and are trafficked like food in the ciliated grooves of the gills.