Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis

• Published in: The Plant journal : for cell and molecular biology Vol. 50; no. 6; pp. 950 - 957
• Main Authors: Devaiah, Shivakumar P, Pan, Xiangqing, Hong, Yueyun, Roth, Mary, Welti, Ruth, Wang, Xuemin
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.06.2007; Blackwell Publishing Ltd; Blackwell Science
• Subjects: Arabidopsis; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - metabolism; Biochemistry; Biological and medical sciences; Botany; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Gene expression; germination; Germination - physiology; Germination and dormancy; lipid oxidation; Molecular biology; Mutagenesis, Insertional; phospholipase D; Phospholipase D - metabolism; Plant physiology and development; polyunsaturated fatty acids; seed deterioration; seed oil content; Seeds; Seeds - enzymology; Seeds - metabolism; Tissue Survival - physiology; phospholipase D; Seeds; seed oil content; Arabidopsis; Germination; polyunsaturated fatty acids; Polyunsaturated fatty acid; Lipids; lipid oxidation; Longevity; Metabolism; Gene; Cruciferae; Dicotyledones; Angiospermae; Unsaturated fatty acid; Spermatophyta; seed deterioration; EC 3.1.4.4
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/j.1365-313X.2007.03103.x

Seed aging decreases the quality of seed and grain and results in agricultural and economic losses. Alterations that impair cellular structures and metabolism are implicated in seed deterioration, but the molecular and biochemical bases for seed aging are not well understood. Ablation of the gene for a membrane lipid-hydrolyzing phospholipase D (PLDα1) in Arabidopsis enhanced seed germination and oil stability after storage or exposure of seeds to adverse conditions. The PLDα1-deficient seeds exhibited a smaller loss of unsaturated fatty acids and lower accumulation of lipid peroxides than did wild-type seeds. However, PLDα1-knockdown seeds were more tolerant of aging than were PLDα1-knockout seeds. The results demonstrate the PLDα1 plays an important role in seed deterioration and aging in Arabidopsis. A high level of PLDα1 is detrimental to seed quality, and attenuation of PLDα1 expression has the potential to improve oil stability, seed quality and seed longevity.