The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle
NCC27 is a nuclear chloride ion channel, identified in the PMA-activated U937 human monocyte cell line. NCC27 mRNA is expressed in virtually all cells and tissues and the gene encoding NCC27 is also highly conserved. Because of these factors, we have examined the hypothesis that NCC27 is involved in...
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Published in | The Journal of physiology Vol. 529; no. 3; pp. 541 - 552 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
The Physiological Society
15.12.2000
Blackwell Science Ltd Blackwell Science Inc |
Subjects | |
Online Access | Get full text |
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Summary: | NCC27 is a nuclear chloride ion channel, identified in the PMA-activated U937 human monocyte cell line. NCC27 mRNA is expressed
in virtually all cells and tissues and the gene encoding NCC27 is also highly conserved. Because of these factors, we have
examined the hypothesis that NCC27 is involved in cell cycle regulation.
Electrophysiological studies in Chinese hamster ovary (CHO-K1) cells indicated that NCC27 chloride conductance varied according
to the stage of the cell cycle, being expressed only on the plasma membrane of cells in G2/M phase.
We also demonstrate that Cl â ion channel blockers known to block NCC27 led to arrest of CHO-K1 cells in the G2/M stage of the cell cycle, the same stage
at which this ion channel is selectively expressed on the plasma membrane.
These data strongly support the hypothesis that NCC27 is involved, in some as yet undetermined manner, in regulation of the
cell cycle. |
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Bibliography: | stella@unsw.edu.au Author's present address S. M. Valenzuela: School of Pathology, The University of New South Wales, Sydney, NSW 2052, Australia. Email ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author’s present address S. M. Valenzuela: School of Pathology, The University of New South Wales, Sydney, NSW 2052, Australia. Email: stella@unsw.edu.au |
ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1111/j.1469-7793.2000.00541.x |