CD146 as a new marker for an increased chondroprogenitor cell sub-population in the later stages of osteoarthritis

• Published in: Journal of orthopaedic research Vol. 33; no. 1; pp. 84 - 91
• Main Authors: Su, Xinlin, Zuo, Wei, Wu, Zhihong, Chen, Jun, Wu, Nan, Ma, Pei, Xia, Zenan, Jiang, Chao, Ye, Zixing, Liu, Sen, Liu, Jiaqi, Zhou, Guangqian, Wan, Chao, Qiu, Guixing
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.01.2015
• Subjects: Adult; Aged; Arthroplasty, Replacement, Knee; Biomarkers - metabolism; Case-Control Studies; CD146; CD146 Antigen - metabolism; Cell Differentiation; Cells, Cultured; Chondrocytes - metabolism; Chondrocytes - pathology; chondrogenesis; chondroprogenitors; Disease Progression; Female; Femur - metabolism; Femur - pathology; Femur - surgery; Humans; Immunophenotyping; Knee Joint - metabolism; Knee Joint - pathology; Knee Joint - surgery; Male; Middle Aged; osteoarthritis; Osteoarthritis, Knee - metabolism; Osteoarthritis, Knee - pathology; Severity of Illness Index; Stem Cells - metabolism; Stem Cells - pathology; chondroprogenitors; chondrogenesis; CD146; osteoarthritis
• ISSN: 0736-0266; 1554-527X; 1554-527X
• DOI: 10.1002/jor.22731

Cartilage‐derived mesenchymal stem cells (MSCs) have been isolated with different methods. In this study lateral and medial femoral condyles were respectively collected from patients with late‐stage osteoarthritis during the total knee arthroplasty. After digestion of the cartilage tissues with type II collagenase and analysis by fluorescence‐activated cell sorting (FACS) with CD146, a chondroprogenitor cell sub‐population were isolated and purified. The expression of other MSC‐associated markers in the CD146+ chondroprogenitors was analyzed by flow cytometry. Multi‐lineage differentiation capacity of CD146+ chondroprogenitors was compared with that of unsorted chondrocytes and adipose‐derived MSCs (ADMSCs). Higher percentage of CD146+ chondroprogenitors isolated from the medial femoral condyles was observed than that from the lateral. CD146+ chondroprogenitors expressed high levels of MSC‐specific surface antigens, and showed higher chondrogenesis capacity than ADMSCs and unsorted chondrocytes in a 3D cell pellet culture model. Thus CD146 might be a new cell surface marker for cartilage progenitor cell population in the late‐stage osteoarthritis. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:84–91, 2015.