Preparation of Specific Antisera to Digoxin Using Hapten-[C-17]-and [C-12]-Bovine Serum Albumin Conjugates

The preparation and antigenic properties of etianic acid-[C-17]-and digoxin-[C-12]-bovine serum albumin (BSA) conjugates are described. The antisera raised against the conjugate having an [O-[N-(carboxymethyl) carbamoyl] methyl] oxime bridge at C-12 in rabbits were highly specific to digoxin, exhibi...

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Published inChemical & pharmaceutical bulletin Vol. 32; no. 6; pp. 2301 - 2306
Main Authors SHIMADA, KAZUTAKE, NAMBARA, TOSHIO, MIZUSAWA, SHIGENORI, OHKUBO, TADASHI
Format Journal Article
LanguageEnglish
Published Tokyo The Pharmaceutical Society of Japan 1984
Maruzen
Japan Science and Technology Agency
Subjects
Analysis
Animals
Biological and medical sciences
Cattle
Chemical Phenomena
Chemistry
Cross Reactions
Digoxin - immunology
digoxin-[C-12]-BSA conjugate
General pharmacology
Haptens
Immune Sera
Medical sciences
Pharmacology. Drug treatments
Rabbits
Serum Albumin, Bovine
Specificity
Antiserum
Radioimmunoassay
Preparation
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ISSN0009-2363
1347-5223
DOI10.1248/cpb.32.2301

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Summary:The preparation and antigenic properties of etianic acid-[C-17]-and digoxin-[C-12]-bovine serum albumin (BSA) conjugates are described. The antisera raised against the conjugate having an [O-[N-(carboxymethyl) carbamoyl] methyl] oxime bridge at C-12 in rabbits were highly specific to digoxin, exhibiting no significant cross-reactions with digoxigenin, or its bis- and monodigitoxosides. The specificity of antisera elicited is discussed in relation to the coupled position of the hapten with BSA.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.32.2301