Target-dependent regulation of acetylcholine secretion at developing motoneurons in Xenopus cell cultures
Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole-cell voltage-clamped myocytes. Changes in SSC amplitude w...
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Published in | The Journal of physiology Vol. 517; no. 3; pp. 721 - 730 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
The Physiological Society
15.06.1999
Blackwell Science Ltd Blackwell Science Inc |
Subjects | |
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Abstract | Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole-cell
voltage-clamped myocytes. Changes in SSC amplitude were assumed to reflect changes in the ACh content of secreted quantal
packets. Compared with natural synapses, motoneurons without any contact with a myocyte (naive neurons) released ACh in smaller
quantal packets.
Bipolar cultured motoneurons, which were in contact with a myocyte with one axon branch (contact-end) but remained free at
another axon branch (free-end), were further used to examine quantal ACh secretion. The ACh quantal size recorded at free-end
terminals was similar to that of naive neurons and was smaller than that at the contact-end, indicating that myocyte contact
exerts differential regulation on quantal secretion in the same neuron.
Some of the neurons that formed a natural synapse with a myocyte continued to grow forward and ACh quantal secretion from
the free growth cone was examined. The ACh quantal size recorded at free growth cones was inversely proportional to the distance
to the natural synapse, implying localized regulation of quantal secretion by the myocyte.
Chronic treatment of day-1 cultures with veratridine and d -tubocurarine, respectively, increased and decreased the neurotrophic action of myocytes when assayed on day 3.
Taken together, these findings suggest that the myocyte is an important postsynaptic target in the regulation of quantal secretion
and that the trophic action is spatially restricted to the neighbourhood of the neuromuscular junction. |
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AbstractList | * 1 Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole-cell voltage-clamped myocytes. Changes in SSC amplitude were assumed to reflect changes in the ACh content of secreted quantal packets. Compared with natural synapses, motoneurons without any contact with a myocyte (naive neurons) released ACh in smaller quantal packets. * 2 Bipolar cultured motoneurons, which were in contact with a myocyte with one axon branch (contact-end) but remained free at another axon branch (free-end), were further used to examine quantal ACh secretion. The ACh quantal size recorded at free-end terminals was similar to that of naive neurons and was smaller than that at the contact-end, indicating that myocyte contact exerts differential regulation on quantal secretion in the same neuron. * 3 Some of the neurons that formed a natural synapse with a myocyte continued to grow forward and ACh quantal secretion from the free growth cone was examined. The ACh quantal size recorded at free growth cones was inversely proportional to the distance to the natural synapse, implying localized regulation of quantal secretion by the myocyte. * 4 Chronic treatment of day-1 cultures with veratridine and d-tubocurarine, respectively, increased and decreased the neurotrophic action of myocytes when assayed on day 3. * 5 Taken together, these findings suggest that the myocyte is an important postsynaptic target in the regulation of quantal secretion and that the trophic action is spatially restricted to the neighbourhood of the neuromuscular junction. Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole-cell voltage-clamped myocytes. Changes in SSC amplitude were assumed to reflect changes in the ACh content of secreted quantal packets. Compared with natural synapses, motoneurons without any contact with a myocyte (naive neurons) released ACh in smaller quantal packets. Bipolar cultured motoneurons, which were in contact with a myocyte with one axon branch (contact-end) but remained free at another axon branch (free-end), were further used to examine quantal ACh secretion. The ACh quantal size recorded at free-end terminals was similar to that of naive neurons and was smaller than that at the contact-end, indicating that myocyte contact exerts differential regulation on quantal secretion in the same neuron. Some of the neurons that formed a natural synapse with a myocyte continued to grow forward and ACh quantal secretion from the free growth cone was examined. The ACh quantal size recorded at free growth cones was inversely proportional to the distance to the natural synapse, implying localized regulation of quantal secretion by the myocyte. Chronic treatment of day-1 cultures with veratridine and d -tubocurarine, respectively, increased and decreased the neurotrophic action of myocytes when assayed on day 3. Taken together, these findings suggest that the myocyte is an important postsynaptic target in the regulation of quantal secretion and that the trophic action is spatially restricted to the neighbourhood of the neuromuscular junction. 1. Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole-cell voltage-clamped myocytes. Changes in SSC amplitude were assumed to reflect changes in the ACh content of secreted quantal packets. Compared with natural synapses, motoneurons without any contact with a myocyte (naive neurons) released ACh in smaller quantal packets. 2. Bipolar cultured motoneurons, which were in contact with a myocyte with one axon branch (contact-end) but remained free at another axon branch (free-end), were further used to examine quantal ACh secretion. The ACh quantal size recorded at free-end terminals was similar to that of naive neurons and was smaller than that at the contact-end, indicating that myocyte contact exerts differential regulation on quantal secretion in the same neuron. 3. Some of the neurons that formed a natural synapse with a myocyte continued to grow forward and ACh quantal secretion from the free growth cone was examined. The ACh quantal size recorded at free growth cones was inversely proportional to the distance to the natural synapse, implying localized regulation of quantal secretion by the myocyte. 4. Chronic treatment of day-1 cultures with veratridine and d-tubocurarine, respectively, increased and decreased the neurotrophic action of myocytes when assayed on day 3. 5. Taken together, these findings suggest that the myocyte is an important postsynaptic target in the regulation of quantal secretion and that the trophic action is spatially restricted to the neighbourhood of the neuromuscular junction. 1 Myocyte‐dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day‐3 Xenopus nerve‐muscle co‐cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole‐cell voltage‐clamped myocytes. Changes in SSC amplitude were assumed to reflect changes in the ACh content of secreted quantal packets. Compared with natural synapses, motoneurons without any contact with a myocyte (naive neurons) released ACh in smaller quantal packets. 2 Bipolar cultured motoneurons, which were in contact with a myocyte with one axon branch (contact‐end) but remained free at another axon branch (free‐end), were further used to examine quantal ACh secretion. The ACh quantal size recorded at free‐end terminals was similar to that of naive neurons and was smaller than that at the contact‐end, indicating that myocyte contact exerts differential regulation on quantal secretion in the same neuron. 3 Some of the neurons that formed a natural synapse with a myocyte continued to grow forward and ACh quantal secretion from the free growth cone was examined. The ACh quantal size recorded at free growth cones was inversely proportional to the distance to the natural synapse, implying localized regulation of quantal secretion by the myocyte. 4 Chronic treatment of day‐1 cultures with veratridine and d‐tubocurarine, respectively, increased and decreased the neurotrophic action of myocytes when assayed on day 3. 5 Taken together, these findings suggest that the myocyte is an important postsynaptic target in the regulation of quantal secretion and that the trophic action is spatially restricted to the neighbourhood of the neuromuscular junction. Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole-cell voltage-clamped myocytes. Changes in SSC amplitude were assumed to reflect changes in the ACh content of secreted quantal packets. Compared with natural synapses, motoneurons without any contact with a myocyte (naive neurons) released ACh in smaller quantal packets. Bipolar cultured motoneurons, which were in contact with a myocyte with one axon branch (contact-end) but remained free at another axon branch (free-end), were further used to examine quantal ACh secretion. The ACh quantal size recorded at free-end terminals was similar to that of naive neurons and was smaller than that at the contact-end, indicating that myocyte contact exerts differential regulation on quantal secretion in the same neuron. Some of the neurons that formed a natural synapse with a myocyte continued to grow forward and ACh quantal secretion from the free growth cone was examined. The ACh quantal size recorded at free growth cones was inversely proportional to the distance to the natural synapse, implying localized regulation of quantal secretion by the myocyte. Chronic treatment of day-1 cultures with veratridine and d -tubocurarine, respectively, increased and decreased the neurotrophic action of myocytes when assayed on day 3. Taken together, these findings suggest that the myocyte is an important postsynaptic target in the regulation of quantal secretion and that the trophic action is spatially restricted to the neighbourhood of the neuromuscular junction. |
Author | Jau-Cheng Liou Yu-Hwa Chen Wen-Mei Fu |
Author_xml | – sequence: 1 givenname: Jau‐Cheng surname: Liou fullname: Liou, Jau‐Cheng – sequence: 2 givenname: Yu‐Hwa surname: Chen fullname: Chen, Yu‐Hwa – sequence: 3 givenname: Wen‐Mei surname: Fu fullname: Fu, Wen‐Mei |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10358113$$D View this record in MEDLINE/PubMed |
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CitedBy_id | crossref_primary_10_1152_jn_90517_2008 crossref_primary_10_1038_nrn1370 crossref_primary_10_1016_S0304_3940_01_01567_1 crossref_primary_10_1016_j_nbd_2005_06_006 crossref_primary_10_1523_JNEUROSCI_23_33_10467_2003 crossref_primary_10_1111_j_1460_9568_2010_07428_x crossref_primary_10_1113_jphysiol_2003_060509 |
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Snippet | Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures.... 1 Myocyte‐dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day‐3 Xenopus nerve‐muscle co‐cultures.... 1. Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures.... * 1 Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures.... Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures.... |
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SubjectTerms | Acetylcholine - metabolism Animals Axons - physiology Cells, Cultured Ciliary Neurotrophic Factor Coculture Techniques Embryo, Nonmammalian Kinetics Motor Neurons - cytology Motor Neurons - drug effects Motor Neurons - physiology Muscles - cytology Muscles - physiology Nerve Growth Factors - pharmacology Nerve Tissue Proteins - pharmacology Neurites - drug effects Neurites - physiology Neuromuscular Junction - physiology Neurotrophin 3 Original Patch-Clamp Techniques Quantum Theory Synapses - drug effects Synapses - physiology Synaptic Transmission - physiology Time Factors Tubocurarine - pharmacology Veratridine - pharmacology Xenopus |
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Title | Target-dependent regulation of acetylcholine secretion at developing motoneurons in Xenopus cell cultures |
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