Selective separation of amino acids with a charged inorganic nanofiltration membrane: Effect of physicochemical parameters on selectivity
A charged organic‐inorganic nanofiltration (NF) membrane prototype was used to separate a mixture of nine amino acids (AA) on the basis of differential electrostatic interactions with the membrane because, for a given pH, some of them were positively charged, some were negative, and some were zwitte...
Saved in:
Published in | Biotechnology and bioengineering Vol. 54; no. 4; pp. 291 - 302 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
20.05.1997
Wiley |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A charged organic‐inorganic nanofiltration (NF) membrane prototype was used to separate a mixture of nine amino acids (AA) on the basis of differential electrostatic interactions with the membrane because, for a given pH, some of them were positively charged, some were negative, and some were zwitterions. Effect of pH, amino acid concentration (Cr), and added ionic strength ([NaCI]) on the process selectivity was studied. A global statistical study revealed that pH was the dominant parameter regarding fractionation. Cr and [NaCI] had a weaker effect, but the ratio Cr/[NaCI] demonstrated a pronounced effect on system selectivity. Two split‐ups of the mixture were obtained at pH 2 and at pH 12, for a 1‐g/L total AA concentration and a Cr/[NaCI] ratio of 0.16. Under these conditions, the differences in transmissions between basic and acid AA were higher than 70%. Interpretation of the results according to the Donnan theory allows us to foresee the potentialities of charged nanofiltration membranes for the fractionation of a complex mixture, such as peptidic hydrolysate to streams containing peptides and amino acids having different isoelectric points. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 291–302, 1997. |
---|---|
Bibliography: | Tech Sep/INRA/ARD - No. 934900044 ark:/67375/WNG-ZK1NG73Z-K Clin Technologies istex:7F9EA94FE10E826A8A90965FA1EF0B76244F9478 ArticleID:BIT1 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0006-3592 1097-0290 |
DOI: | 10.1002/(SICI)1097-0290(19970520)54:4<291::AID-BIT1>3.0.CO;2-F |