Methicillin-resistant Staphylococcus aureus (MRSA) with reduced susceptibility to glycopeptides (GISA) in 63 French general hospitals

• Published in: Clinical microbiology and infection Vol. 10; no. 5; pp. 448 - 451
• Main Authors: Cartolano, G.L., Cheron, M., Benabid, D., Leneveu, M., Boisivon, A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.05.2004; Blackwell Science Ltd; Blackwell
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Bacterial diseases; Biological and medical sciences; Drug Resistance, Bacterial; Electrophoresis, Gel, Pulsed-Field; France - epidemiology; Fundamental and applied biological sciences. Psychology; glycopeptides; Human bacterial diseases; Humans; Infectious diseases; Medical sciences; Methicillin Resistance; Microbial Sensitivity Tests; Microbiology; resistance; Staphylococcal Infections - drug therapy; Staphylococcal Infections - epidemiology; Staphylococcal Infections - microbiology; Staphylococcal infections, streptococcal infections, pneumococcal infections; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - isolation & purification; teicoplanin; Teicoplanin - pharmacology; Teicoplanin - therapeutic use; vancomycin; Vancomycin - pharmacology; Vancomycin - therapeutic use; Vancomycin Resistance; France; Europe; teicoplanin; vancomycin; glycopeptides; Staphylococcus aureus; resistance; Microbiology; Drug susceptibility test; Infection; Glycopeptide; Bacteriosis; Bacteria; Micrococcales; Micrococcaceae; Hospital; Staphylococcal infection
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2004.00830.x

Clinical isolates of methicillin-resistant Staphylococcus aureus (n = 1070) collected from 63 French general hospitals during June 2000 (n = 1070) were screened initially for reduced susceptibility to glycopeptides (GISA) on brain–heart infusion agar containing teicoplanin 6 mg/L. Glycopeptide MICs were determined for the 145 isolates that grew on the screening plates. Of the 1070 isolates, 1.4% were GISA on Mueller–Hinton agar, and 2.9% by Etest with a high inoculum, while 0.7% and 2.9% were GISA by vancomycin and teicoplanin population analysis profiles, respectively. Most isolates were resistant to gentamicin and rifampicin or fosfomycin or fusidic acid, as determined by disk diffusion. Pulsed-field gel electrophoresis of the 31 GISA isolates identified four clones, with dissemination of one predominant clone. In these French hospitals there was a low incidence of GISA and hetero-GISA.