Antioxidative response to Cd in a newly discovered cadmium hyperaccumulator, Arabis paniculata F

• Published in: Chemosphere (Oxford) Vol. 74; no. 1; pp. 6 - 12
• Main Authors: Qiu, Rong-Liang, Zhao, Xuan, Tang, Ye-Tao, Yu, Fang-Ming, Hu, Peng-Jie
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.2008; Elsevier
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Antioxidative defense; Arabis; Arabis - drug effects; Arabis - metabolism; Arabis paniculata F; Ascorbate Oxidase - metabolism; Autoecology; Biological and medical sciences; Cadmium; Cadmium - metabolism; Cadmium - toxicity; Fundamental and applied biological sciences. Psychology; Glutathione Reductase - metabolism; Hydrogen Peroxide - metabolism; Hyperaccumulator; Lipid Peroxidation - drug effects; Plant Roots - drug effects; Plant Roots - metabolism; Plant Shoots - drug effects; Plant Shoots - metabolism; Plants and fungi; Reactive Oxygen Species - metabolism; ROS; Superoxides - metabolism; Cadmium; Antioxidative defense; Arabis paniculata F; Hyperaccumulator; ROS; Antioxidant; Heavy metal; Pollutant; Plant; Cruciferae; Dicotyledones; Angiospermae; Defense mechanism; Spermatophyta
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2008.09.069

A hydroponic experiment was carried out to study the effect of cadmium (Cd) on growth, Cd accumulation, lipid peroxidation, reactive oxygen species (ROS) content and antioxidative enzymes in leaves and roots of Arabis paniculata F., a new Cd hyperaccumuator found in China. The results showed that 22–89 μM Cd in solution enhanced the growth of A. paniculata after three weeks, with 21–27% biomass increase compared to the control. Cd concentrations in shoots and roots increased with increasing Cd supply levels, and reached a maximum of 1662 and 8670 mg kg −1 Cd dry weight at 178 μM Cd treatment, respectively. In roots, 22–89 μM Cd reduced the content of malondialdehyde (MDA), superoxide ( O 2 - 1 ) and H 2O 2 as well as the activities of superoxide dismutase (SOD), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR). In leaves, the contents of MDA, O 2 - 1 and H 2O 2 remained unaffected by 22–89 μM Cd, while 178 μM Cd treatment significantly increased the MDA content, 69.5% higher than that of the control; generally, the activities of SOD, catalase (CAT), GPX and APX showed an increasing pattern with increasing Cd supply levels. Our present work concluded that A. paniculata has a great capability of Cd tolerance and accumulation. Moderate Cd treatment (22–89 μM Cd) alleviated the oxidative stress in roots, while higher level of Cd addition (178 μM) could cause an increasing generation of ROS, which was effectively scavenged by the antioxidative system.