Directed Evolution of Streptomyces clavuligerus Deacetoxycephalosporin C Synthase for Enhancement of Penicillin G Expansion

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Published inApplied and Environmental Microbiology Vol. 71; no. 12; pp. 8873 - 8880
Main Authors WEI, Chia-Li, YANG, Yunn-Bor, DENG, Chan-Hui, LIU, Wen-Chi, HSU, Jyh-Shing, LIN, Yu-Ching, LIAW, Shwu-Huey, TSAI, Ying-Chieh
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.12.2005
Subjects
Amino Acid Sequence
Amino Acid Substitution
Antibiotics
Bacteria
Biological and medical sciences
Biotechnology
Directed Molecular Evolution - methods
Enzymes
Enzymology and Protein Engineering
Evolution
Fundamental and applied biological sciences. Psychology
Intramolecular Transferases - chemistry
Intramolecular Transferases - genetics
Intramolecular Transferases - metabolism
Methods. Procedures. Technologies
Microbiology
Mutagenesis, Site-Directed
Mutation
Penicillin
Penicillin G - metabolism
Penicillin-Binding Proteins - chemistry
Penicillin-Binding Proteins - genetics
Penicillin-Binding Proteins - metabolism
Penicillins - biosynthesis
Polymerase Chain Reaction
Protein Conformation
Protein engineering
Proteins
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Streptomyces - enzymology
Streptomyces - genetics
Streptomyces clavuligerus
Actinomycetales
Directed molecular evolution
Antibiotic
Streptomycetaceae
Bacteria
Actinomycetes
Benzylpenicillin
Streptomyces clavuligerus
Mutation
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Summary:Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to AEM .asm.org, visit: AEM       
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content type line 23
Corresponding author. Mailing address: Institute of Biochemistry, National Yang-Ming University, 155, Sec. 2, Li-Nong St., Pei-Tou, Taipei 11221, Taiwan. Phone: (886) 2-2826-7125. Fax: (886) 2-2826-4843. E-mail for S.-H. Liaw: shliaw@ym.edu.tw. E-mail for Y.-C. Tsai: tsaiyc@ym.edu.tw.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.12.8873-8880.2005