Type I-Secreted, Sulfated Peptide Triggers XA21-Mediated Innate Immunity

The rice Xa21 gene confers immunity to most strains of the bacterium Xanthomonas oryzae pv. oryzae (Xoo). Liquid chromatography-tandem mass spectrometry analysis of biologically active fractions from Xoo supernatants led to the identification of a 194-amino acid protein designated Ax21 (activator of...

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Published inScience (American Association for the Advancement of Science) Vol. 326; no. 5954; pp. 850 - 853
Main Authors Lee, Sang-Won, Han, Sang-Wook, Sririyanum, Malinee, Park, Chang-Jin, Seo, Young-Su, Ronald, Pamela C
Format Journal Article
LanguageEnglish
Published Washington, DC American Association for the Advancement of Science 06.11.2009
The American Association for the Advancement of Science
Subjects
Bacteria
Bacterial plant pathogens
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control
Immunity
Immunology
Innate immunity
Inoculation
Lesions
Microorganisms
Molecular biology
Molecules
Oryza sativa
Peptides
Phytopathology. Animal pests. Plant and forest protection
Proteins
Rice
Sulfation
Xanthomonas
Xanthomonas oryzae
Pseudomonadales
Monocotyledones
Natural immunity
Host agent relation
Pathogen Associated Molecular Patterns
Cereal crop
Oryza sativa
Gramineae
Angiospermae
Bacteria
Pseudomonadaceae
Spermatophyta
Xanthomonas oryzae pv. oryzae
C3-Type
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Summary:The rice Xa21 gene confers immunity to most strains of the bacterium Xanthomonas oryzae pv. oryzae (Xoo). Liquid chromatography-tandem mass spectrometry analysis of biologically active fractions from Xoo supernatants led to the identification of a 194-amino acid protein designated Ax21 (activator of XA21-mediated immunity). A sulfated, 17-amino acid synthetic peptide (axYS22) derived from the N-terminal region of Ax21 is sufficient for activity, whereas peptides lacking tyrosine sulfation are biologically inactive. Using coimmunoprecipitation, we found that XA21 is required for axYS22 binding and recognition. axYS22 is 100% conserved in all analyzed Xanthomonas species, confirming that Ax21 is a pathogen-associated molecular pattern and that XA21 is a pattern recognition receptor.
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ISSN:0036-8075
1095-9203
DOI:10.1126/science.1173438