Genetic Variants Regulating Immune Cell Levels in Health and Disease
The complex network of specialized cells and molecules in the immune system has evolved to defend against pathogens, but inadvertent immune system attacks on “self” result in autoimmune disease. Both genetic regulation of immune cell levels and their relationships with autoimmunity are largely undet...
Saved in:
Published in | Cell Vol. 155; no. 1; pp. 242 - 256 |
---|---|
Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
26.09.2013
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The complex network of specialized cells and molecules in the immune system has evolved to defend against pathogens, but inadvertent immune system attacks on “self” result in autoimmune disease. Both genetic regulation of immune cell levels and their relationships with autoimmunity are largely undetermined. Here, we report genetic contributions to quantitative levels of 95 cell types encompassing 272 immune traits, in a cohort of 1,629 individuals from four clustered Sardinian villages. We first estimated trait heritability, showing that it can be substantial, accounting for up to 87% of the variance (mean 41%). Next, by assessing ∼8.2 million variants that we identified and confirmed in an extended set of 2,870 individuals, 23 independent variants at 13 loci associated with at least one trait. Notably, variants at three loci (HLA, IL2RA, and SH2B3/ATXN2) overlap with known autoimmune disease associations. These results connect specific cellular phenotypes to specific genetic variants, helping to explicate their involvement in disease.
[Display omitted]
•Immune system analysis of 95 distinct cell types using flow cytometry and GWAS•An unexpectedly large genetic component drives variation in immune cell numbers•Sequencing-based GWAS reveals new immune cell trait-associated SNPs•Immune trait-disease overlapping associations point to specific cells as risk factors
To analyze the role of genetics in immunity regulation and immune cell numbers, a combination of flow cytometry and GWA analyses profiles 95 cell types of the innate and adaptive immune system to connect specific cellular phenotypes to immune-related diseases predisposition. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work |
ISSN: | 0092-8674 1097-4172 |
DOI: | 10.1016/j.cell.2013.08.041 |