Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals

• Published in: Vaccine Vol. 29; no. 34; pp. 5688 - 5693
• Main Authors: Rohani, Md Yasin, Zin, Norni Mohd, Hussin, Azura, Nawi, Salbiah Haji, Hanapiah, Suhailah Md, Wahab, Zubaidah Abdul, Raj, Ganeswarie, Shafie, Norazita, Peng, Ng Pey, Chu, Keah K, Aziz, Muhhamad Nazri, Maning, Nurahan, Mohamad, Jamilah Said, Benjamin, Adom, Salleh, Mohd. Azmi bin Mohd, Zahari, Sabarina Sabihah, Francis, Alex, Ahmad, Norazah, Karunakaran, Rina
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 05.08.2011; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Aged; Allergy and Immunology; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Antibiotics; Applied microbiology; Bacteriology; Biological and medical sciences; blood; Child; Child, Preschool; children; Children & youth; Community-Acquired Infections - microbiology; Disk Diffusion Antimicrobial Tests; Drug Resistance, Bacterial; Female; Fundamental and applied biological sciences. Psychology; hospitals; Humans; Infant; Malaysia; Male; Meningitis; Microbial Sensitivity Tests; Microbiology; Middle Aged; minimum inhibitory concentration; Miscellaneous; patients; Penicillin; Pneumococcal Infections; Pneumococcal serotype; Pneumococcal susceptibility pattern; Pneumococcal vaccine; Pneumonia; Pneumonia - microbiology; serotypes; Serotyping; Streptococcus pneumoniae; Streptococcus pneumoniae - classification; Streptococcus pneumoniae - drug effects; Streptococcus pneumoniae - immunology; Streptococcus pneumoniae - isolation & purification; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Malaysia; United States > US; Pneumococcal serotype; Pneumococcal vaccine; Pneumococcal susceptibility pattern; Streptococcaceae; Antibiotic; Sensitivity; Bacteria; Micrococcales; Vaccine; Serotype; Streptococcus pneumoniae
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2011.06.004

Abstract From January 2008 to December 2009, 433 Streptococcus pneumoniae strains were examined to determine the serotype distribution and susceptibility to selected antibiotics. About 50% of them were invasive isolates. The strains were isolated from patients of all age groups and 33.55% were isolated from children below 5 years. The majority was isolated from blood (48.53%) and other sterile specimens (6.30%). Community acquired pneumonia (41.70%) is the most common diagnosis followed by sepsis (9.54%). Serotyping was done using Pneumotest Plus-Kit and antibiotic susceptibility pattern was determined by modified Kirby–Bauer disk diffusion method and measurement of minimum inhibitory concentration (MIC) using E -test strip. Ten most common serotypes were 19F (15.02%), 6B (10.62%), 19A (6.93%), 14 (6.70%), 1 (5.08%), 6A (5.08%), 23F (4.85%), 18C (3.93%), 3 (2.08%) and 5 (1.85%). Penicillin MIC ranged between ≤0.012–4 μg/ml with MIC90 of 1 μg/ml. Penicillin resistant rate is 31.78%. The majority of penicillin less-susceptible strains belonged to serotype 19F followed by 19A and 6B. Based on the serotypes distribution 22 (44.00%), 28 (56.00%) and 39 (78.00%) of the invasive isolates from children ≤2 years were belonged to serotypes included in the PCV7, PCV10 and PCV13, respectively.