miR-300/FA2H affects gastric cancer cell proliferation and apoptosis

• Published in: Open medicine (Warsaw, Poland) Vol. 15; no. 1; pp. 882 - 889
• Main Authors: Hong, Bo, Li, Jie, Huang, Chunxiao, Huang, Tao, Zhang, Mengpei, Huang, Lijiang
• Format: Journal Article
• Language: English
• Published: Poland De Gruyter 01.01.2020; Walter de Gruyter GmbH
• Subjects: Apoptosis; cell apoptosis; Cell growth; cell proliferation; fatty acid 2-hydroxylase; Gastric cancer; microRNA-300; MicroRNAs; cell apoptosis; fatty acid 2-hydroxylase; microRNA-300; cell proliferation; gastric cancer
• ISSN: 2391-5463; 2391-5463
• DOI: 10.1515/med-2020-0188

MicroRNA (miR/miRNA) expression disorders play a crucial role in the development of gastric cancer (GC). Increasing evidence has indicated that miRNAs participate in the process of numerous cancers. Previous research has demonstrated that miR-300 acts as a cancer-promoting factor or tumor suppressor in a number of tumors. However, to the best of our knowledge, the effects of miR-300 on GC cells remain largely unknown. The present study investigated the effects of miR-300 on GC cells and analyzed its molecular mechanism. First, reverse transcription–quantitative polymerase chain reaction showed that miR-300 expression was increased in GC tissues and cell lines, with the highest expression observed in human gastric cancer cell line AGS. Subsequent results indicated that fatty acid 2-hydroxylase (FA2H) was a target of miR-300. FA2H-plasmid inhibited AGS cell proliferation and induced apoptosis. Finally, miR-300 inhibitor reduced cell proliferation and induced apoptosis, whereby these effects were reversed by FA2H-small interfering RNA. Therefore, the data demonstrated that miR-300/FA2H might be a new potential biomarker and therapeutic target for GC treatment.