In vivo X-ray cine-tomography for tracking morphological dynamics
Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information i...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 111; no. 11; pp. 3921 - 3926 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
18.03.2014
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and sub-second time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut—type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by Stefan Hau-Riege, Lawrence Livermore National Lab, Livermore, CA, and accepted by the Editorial Board February 10, 2014 (received for review May 7, 2013) Author contributions: T.d.S.R., T.v.d.K., and T.B. designed research; T.d.S.R., A.E., and T.v.d.K. performed research; T.d.S.R., A.E., and T.v.d.K. analyzed data; T.B. supervised research; and T.d.S.R., A.E., T.v.d.K., and T.B. wrote the paper. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.1308650111 |