Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa

• Published in: Scientific reports Vol. 10; no. 1; pp. 11040 - 17
• Main Authors: Crizel, Rosane Lopes, Perin, Ellen Cristina, Vighi, Isabel Lopes, Woloski, Rafael, Seixas, Amilton, da Silva Pinto, Luciano, Rombaldi, César Valmor, Galli, Vanessa
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 06.07.2020; Nature Publishing Group UK; Nature Portfolio
• Subjects: Abiotic stress; Abscisic acid; Abscisic Acid - pharmacology; Calcium Signaling; Calcium signalling; Cellular stress response; Chromosome Mapping; Chromosomes, Plant - genetics; Drought; Fragaria - genetics; Fragaria - growth & development; Fragaria - metabolism; Fragaria ananassa; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Plant - drug effects; Genes; Genome, Plant; Genomes; Kinases; Multigene Family; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Interaction Maps; Protein kinase; Protein Kinases - genetics; Protein Kinases - metabolism; Ribonucleic acid; RNA; RNA-Seq; Stress, Physiological - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-67957-9

Calcium-dependent protein kinases (CDPKs) are encoded by a large gene family and play important roles against biotic and abiotic stresses and in plant growth and development. To date, little is known about the CDPK genes in strawberry (Fragaria x ananassa). In this study, analysis of Fragaria x ananassa CDPK gene family was performed, including gene structures, phylogeny, interactome and expression profiles. Nine new CDPK genes in Fragaria x ananassa were identified based on RNA-seq data. These identified strawberry FaCDPK genes were classified into four main groups, based on the phylogenetic analysis and structural features. FaCDPK genes were differentially expressed during fruit development and ripening, as well as in response to abiotic stress (salt and drought), and hormone (abscisic acid) treatment. In addition, the interaction network analysis pointed out proteins involved in the ABA-dependent response to plant stress via Ca signaling, especially RBOHs. To our knowledge, this is the first report on CDPK families in Fragaria x ananassa, and it will provide valuable information for development of biofortified fruits and stress tolerant plants.