In vitro antimicrobial activity of Millettia laurentii De Wild and Lophira alata Banks ex C. F. Gaertn on selected foodborne pathogens associated to gastroenteritis

This study aimed at evaluating the antimicrobial potential of aqueous, ethanolic and methanolic extracts of two Cameroonian plants against selected foodborne pathogens. Bioactive compounds were extracted from Millettia laurentii De Wild seeds and Lophira alata Banks ex. C. F. Gaertn leaves using dis...

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Published inHeliyon Vol. 7; no. 4; p. e06830
Main Authors Mouafo, Hippolyte Tene, Tchuenchieu, Alex Dimitri Kamgain, Nguedjo, Maxwell Wandji, Edoun, Ferdinand Lanvin Ebouel, Tchuente, Boris Ronald Tonou, Medoua, Gabriel Nama
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.04.2021
Elsevier
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Summary:This study aimed at evaluating the antimicrobial potential of aqueous, ethanolic and methanolic extracts of two Cameroonian plants against selected foodborne pathogens. Bioactive compounds were extracted from Millettia laurentii De Wild seeds and Lophira alata Banks ex. C. F. Gaertn leaves using distilled water, ethanol and methanol as solvents. The extracts were tested against Escherichia coli O157, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Moraxella morganii, Salmonella enteritidis, Klebsiella pneumoniae and Listeria monocytogenes using the microdilution method. The results showed that distilled water extracted a more important mass of phytochemical compounds (18.0–24.60%) compared to ethanol (4.80–5.0%) and methanol (4.20–4.60%). All the extracts exhibited significant antimicrobial activity with MIC values ranging from 5 to 20 μg/mL for M. laurentii seeds extracts and from 1.0 to 20 μg/mL for L. alata leaves extracts. The different plant extracts were ten times less active than gentamicin. The most active extracts were obtained using ethanol as solvent and K. pneumoniae was the most resistant pathogen to all extracts (MBC>20 μg/mL). M. laurentii extracts were bactericidal against L. monocytogenes and P. mirabilis while the reference antibiotic (gentamicin) was bacteriostatic against these pathogens. The results obtained from this study suggest the studied local plant materials as a source of antimicrobial compounds which can be valorized in the medical field as substitute of antibiotics for which many microorganisms have nowadays developed resistance mechanisms. Further studies need to be performed in order to characterize and identify these antimicrobial active molecules. Millettia laurentii seeds, Lophira alata leaves, Extraction solvents, Antimicrobial activity, Foodborne pathogens.
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ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2021.e06830