Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 51; no. 3; pp. 985 - 997
• Main Authors: Barbeyron, T, L'Haridon, S, Corre, E, Kloareg, B, Potin, P
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Reading Soc General Microbiol 01.05.2001; Society for General Microbiology; Microbiology Society
• Subjects: Anti-Bacterial Agents - pharmacology; Bacteriology; Biological and medical sciences; Cytophaga - classification; Cytophaga - drug effects; Cytophaga - genetics; Cytophaga - isolation & purification; Cytophaga uliginosa; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Escherichia coli - genetics; Fatty Acids - analysis; Flavobacteriaceae; Flavobacterium - classification; Flavobacterium - drug effects; Flavobacterium - genetics; Flavobacterium - isolation & purification; France; Fundamental and applied biological sciences. Psychology; Life Sciences; Microbial Sensitivity Tests; Microbiology; Microbiology and Parasitology; Molecular Sequence Data; Mycology; Nucleic Acid Hybridization; Phenotype; Phylogeny; Rhodophyta - microbiology; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seawater - microbiology; Systematics; Zobellia galactanovorans; Zobellia uliginosa; France; Red Sea; Europe; Ability; Flavobacteriaceae; Taxonomy; Algae; Agar; Taxonomic review; Cytophagaceae; Classification; Mesophily; Bacteria; Colony; Isolate; Isolation; Carrageenan; Cytophagales; Cell; Cytophaga; Species; Thallophyta; Zobellia galactanovorans; carrageenase and agarase activities; Zobellia uliginosa; [Cytophaga] uliginosa
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/00207713-51-3-985

T Barbeyron, S L'Haridon, E Corre, B Kloareg and P Potin Station Biologique de Roscoff, UMR 1931 (CNRS and Laboratoire Goemar), Place Georges Teissier, 29682 Roscoff Cedex, Bretagne, France A mesophilic, aerobic, non-flagellated, gliding bacterium, forming yellow colonies and designated Dsij(T), was isolated from a red alga on the sea-shore of Roscoff, Brittany, France. Dsij(T) was selected for its ability to actively degrade both agars and carrageenans. The Gram-negative cells occurred singly or in pairs as long rods. The temperature range for growth was 13--45 degrees C, with an optimum at 35 degrees C. The pH range for growth at 35 degrees C was from 6.0 to 8.5, with an optimum around pH 7.0. The NaCl concentrations required for growth at 35 degrees C and pH 7.0 ranged from 5 to 60 g l(-1), with an optimum around 25 g l(-1). The G+C content of the genomic DNA was 42--43 mol%. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain Dsij(T) is closely related to [Cytophaga] uliginosa DSM 2061(T). Phenotypic features, however, allowed Dsij(T) and [Cytophaga] uliginosa strains to be distinguished on the basis of ten traits (spreading behaviour, assimilation of eight compounds and amylase production). Their total protein profiles were also different and DNA--DNA hybridization experiments confirmed that Dsij(T) constitutes a new species, distinct from [Cytophaga] uliginosa. Based on the phenotypic features and the phylogenetic relationships of the Flavobacteriaceae, a new genus designated Zobellia gen. nov. is proposed to include Zobellia galactanovorans gen. nov., sp. nov., while [Cytophaga] uliginosa becomes Zobellia uliginosa comb. nov. The type strain of Zobellia galactanovorans is Dsij(T) (=DSM 12802(T)=CIP 106680(T)).