Tubulin and actin topology during zygote formation of Saccharomyces cerevisiae

• Published in: Journal of general microbiology Vol. 133; no. 12; pp. 3355 - 3363
• Main Authors: Hasek, J, Rupes, I, Svobodova, J, Streiblova, E
• Format: Journal Article
• Language: English
• Published: London Soc General Microbiol 01.12.1987; New York, NY Cambridge University Press
• Subjects: actin; Actins - analysis; Benzimidazoles - pharmacology; Biological and medical sciences; Cell Cycle; Conjugation, Genetic - drug effects; Fundamental and applied biological sciences. Psychology; Microbiology; Microscopy, Fluorescence; monoclonal antibodies; morphogenesis; Morphology, structure, chemical composition; Mycology; Nocodazole; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - ultrastructure; Tubulin - analysis; zygote; Zygote - ultrastructure; Fungi; Morphogenesis; Proteins; Yeast; Tubulin; Zygote; Cell cycle; Actins; Cytoskeleton; Ascomycetes; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0022-1287; 1350-0872; 1465-2080
• DOI: 10.1099/00221287-133-12-3355

Institute of Microbiology, Czechoslovak Academy of Sciences, Vide ská 1083, 142 20 Prague 4, Czechoslovakia ABSTRACT SUMMARY: The topology of tubulin and actin during mating of Saccharomyces cerevisiae was analysed by fluorescence microscopy with the monoclonal anti-tubulin antibody TuO1 and rhodamine-labelled phalloidin. Preconjugatory cells displayed an asymmetric distribution of the microtubule and actin cytoskeleton and an overall polarization of the cells preceding cell fusion. Prior to karyogamy, the haploid spindle pole bodies were associated with abundant cytoplasmic microtubules. Budding zygotes revealed the same tubulin and actin patterns as vegetative cells. Treatment of the mating mixture with the microtubule inhibitor nocodazole (10 µg ml -1 ) did not prevent polarization and fusion of haploids, zygote formation and emergence of the first zygotic bud. In marked contrast, the migration of the nucleus in preconjugatory cells as well as nuclear migration and fusion within the zygotes was unequivocally blocked by the action of the drug. It is suggested that the problem of the morphogenesis of mating should be approached by considering interactions at the cell periphery.