Illumina MiSeq Analysis and Comparison of Freshwater Microalgal Communities on Ulleungdo and Dokdo Islands

• Published in: Polish journal of microbiology Vol. 68; no. 4; pp. 527 - 539
• Main Authors: Yun, Hyun-Sik, Kim, Young-Saeng, Yoon, Ho-Sung
• Format: Journal Article
• Language: English
• Published: Poland De Gruyter Poland 01.12.2019; Exeley Inc; Sciendo
• Subjects: Algae; amplicon sequencing; Aquatic microorganisms; Aquatic plants; Bacillariophyta; Chlorophyta; Chlorophyta - classification; Chlorophyta - genetics; Diatoms - classification; Diatoms - genetics; Diatoms - isolation & purification; Dokdo Island; Ecosystems; Environmental conditions; Flowers & plants; Fresh Water - analysis; Freshwater resources; Genera; Groundwater; Islands; Marine microorganisms; Microalgae - classification; Microalgae - genetics; Microalgae - isolation & purification; microalgal community; Microbial activity; Microbiology; Microorganisms; MiSeq system; Phylogeny; Precipitation; Sequence Analysis, DNA; Species richness; Systems analysis; Taxonomy; Ulleungdo Island; Vegetation; Volcanic islands; South Korea
• ISSN: 2544-4646; 1733-1331; 2544-4646
• DOI: 10.33073/pjm-2019-053

Ulleungdo and Dokdo are volcanic islands with an oceanic climate located off the eastern coast of South Korea. In the present study, we used barcoded Illumina MiSeq to analyze eukaryotic microalgal genera collected from Seonginbong, the highest peak on Ulleungdo, and from groundwater sites on Dongdo and Seodo Islands, which are part of Dokdo. Species richness was significantly greater in the Seonginbong samples than in the Dongdo and Seodo samples, with 834 operational taxonomic units (OTUs) identified from Seonginbong compared with 203 OTUs and 182 OTUs from Dongdo and Seodo, respectively. Taxonomic composition analysis was also used to identify the dominant microalgal phyla at each of the three sites, with Chlorophyta (green algae) the most abundant phyla on Seonginbong and Dongdo, and Bacillariophyta (diatoms) the most abundant on Seodo. These findings suggest that differences in the abundances of Chlorophyta and Bacillariophyta species in the Seonginbong, Dongdo, and Seodo samples are due to variations in species richness and freshwater resources at each sampling location. To the best of our knowledge, this is the first report to detail freshwater microalgal communities on Ulleungdo and Dokdo. As such, the number of species identified in the Seonginbong, Dongdo, and Seodo samples might be an indicator of the ecological differences among these sites and varying characteristics of their microbial communities. Information regarding the microalgal communities also provides a basis for understanding the ecological interactions between microalgae species and other eukaryotic microorganisms. Ulleungdo and Dokdo are volcanic islands with an oceanic climate located off the eastern coast of South Korea. In the present study, we used barcoded Illumina MiSeq to analyze eukaryotic microalgal genera collected from Seonginbong, the highest peak on Ulleungdo, and from groundwater sites on Dongdo and Seodo Islands, which are part of Dokdo. Species richness was significantly greater in the Seonginbong samples than in the Dongdo and Seodo samples, with 834 operational taxonomic units (OTUs) identified from Seonginbong compared with 203 OTUs and 182 OTUs from Dongdo and Seodo, respectively. Taxonomic composition analysis was also used to identify the dominant microalgal phyla at each of the three sites, with Chlorophyta (green algae) the most abundant phyla on Seonginbong and Dongdo, and Bacillariophyta (diatoms) the most abundant on Seodo. These findings suggest that differences in the abundances of Chlorophyta and Bacillariophyta species in the Seonginbong, Dongdo, and Seodo samples are due to variations in species richness and freshwater resources at each sampling location. To the best of our knowledge, this is the first report to detail freshwater microalgal communities on Ulleungdo and Dokdo. As such, the number of species identified in the Seonginbong, Dongdo, and Seodo samples might be an indicator of the ecological differences among these sites and varying characteristics of their microbial communities. Information regarding the microalgal communities also provides a basis for understanding the ecological interactions between microalgae species and other eukaryotic microorganisms.