One-pot synthesis of zinc oxide nanoparticles via chemical precipitation for bromophenol blue adsorption and the antifungal activity against filamentous fungi

• Published in: Scientific reports Vol. 11; no. 1; pp. 8305 - 17
• Main Authors: Akpomie, Kovo G, Ghosh, Soumya, Gryzenhout, Marieka, Conradie, Jeanet
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 15.04.2021; Nature Publishing Group UK; Nature Portfolio
• Subjects: Adsorption; Alternaria alternata; Antifungal activity; Antifungal agents; Bromphenol Blue - chemistry; Chemical Precipitation; Drug Resistance, Fungal; Fungi; Fungi - drug effects; Nanoparticles; Thermal stability; Zinc oxide; Zinc Oxide - chemical synthesis; Zinc Oxide - pharmacology; Zinc oxides
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-87819-2

In this research, zinc oxide nanoparticles (ZnONPs) were prepared via a facile one-pot chemical precipitation approach and applied in the adsorption of bromophenol blue (BRB) and as antifungal agents against the filamentous fungi and plant pathogens; Alternaria alternata CGJM3078, Alternaria alternata CGJM3006 and Fusarium verticilliodes CGJM3823. The ZnONPs were characterized by the UV-Vis, FTIR, XRD, TGA, BET, SEM, TEM, and EDX techniques, which showed efficient synthesis. The characteristics ZnO UV-Vis absorption band was observed at 375 nm, while the XRD showed an average ZnONPs crystalline size of 47.2 nm. The SEM and TEM images showed an irregular shaped and aggregated porous structure of 65.3 nm average-sized ZnONPs. The TGA showed 22.9% weight loss at 800 °C indicating the high thermal stability of ZnONPs, while BET analysis revealed a surface area, pore volume and pore diameter of 9.259 m /g, 0.03745 cm /g and 9.87 nm respectively. The Freundlich, pseudo-second-order, and intra-particle diffusion models showed R  > 0.9494 and SSE < 0.7412, thus, exhibited the best fit to the isotherm and kinetics models. Thermodynamics revealed feasible, endothermic, random, and spontaneous adsorption of BRB onto the synthesized ZnONPs. The antifungal assay conducted depicts strong antifungal activities against all three tested fungi. Noticeably, ZnONPs (0.002-5 mg/mL) showed maximum activities with the largest zone of inhibition against A. alternata CGJM 3006 from 25.09 to 36.28 mm. This was followed by the strain F. verticilliodes CGJM 3823 (range from 23.77 to 34.77 mm) > A. alternata CGJM3078 (range from 22.73 to 30.63 mm) in comparison to Bleach 5% (positive control). Additionally a model was proposed based on the possible underlying mechanisms for the antifungal effect. This research demonstrated the potent use of ZnONPs for the adsorption of BRB and as effective antifungal agents.