Preparation of Polyoxometalate-Based Composite by Solidification of Highly Active Cobalt-Containing Polytungstate on Polymeric Ionic Liquid for the Efficient Isolation of Proteinase K

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 8; p. 3307
• Main Authors: Yang, Jiaxuan, Chu, Ning, Chen, Xuwei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.04.2023; MDPI
• Subjects: Adsorption; Amino acids; Ammonium; Ammonium chloride; Aspartic acid; Bonding strength; Carbon dioxide; Cation exchanging; Chemical bonds; Cobalt; Endopeptidase K; Free radicals; Hydrocarbons; Hydrogen bonding; Ionic liquids; Ions; isolation; Oxidation; Peptides; Polyethylenes; polymeric ionic liquid; Polymerization; polyoxometalate; Polyoxometallates; Polyvinyl alcohol; Proteinase; proteinase K (PrK); Solidification; water-soluble polytungstate; isolation; polymeric ionic liquid; proteinase K (PrK); water-soluble polytungstate; polyoxometalate
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28083307

A novel porous polyoxometalate (POM)-based composite (Co PW-PDDVAC) was prepared via the solidification of water-soluble polytungstate (Co PW) on the polymeric ionic liquid dimethyldodecyl-4-polyethylene benzyl ammonium chloride (PDDVAC) via a cation-exchange reaction. The solidification was confirmed by EDS, SEM, FT-IR, TGA, and so on. The strong covalent coordination and hydrogen-bonding interaction between the highly active Co of the Co PW and the aspartic acid residues of proteinase K endowed the obtained Co PW-PDDVAC composite with excellent proteinase K adsorption properties. Thermodynamic investigations indicate that the adsorption behavior of proteinase K was consistent with the linear Langmuir isothermal model, giving an adsorption capacity as high as 1428 mg g . The Co PW-PDDVAC composite was applied in the selective isolation of highly active proteinase K from Limber crude enzyme fluid.