Mutagenicity Tests with a Permeable Mutant of Yeast on Carcinogens Showing False-Negative in Salmonella Assay

• Published in: Chemical & pharmaceutical bulletin Vol. 37; no. 2; pp. 407 - 409
• Main Authors: SHIMIZU, Tadayori, MORITA, Tamotsu, IWAMOTO, Yoshihisa, YANAGIHARA, Yasutake, MASUZAWA, Toshiyuki
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.02.1989; Maruzen; Japan Science and Technology Agency
• Subjects: Animals; Biological and medical sciences; carcinogen; Carcinogenesis, carcinogens and anticarcinogens; Carcinogens - toxicity; cell permeability; Chemical agents; false-negative; In Vitro Techniques; Medical sciences; mutagenicity test; Mutagenicity Tests; Mutagens; permeable mutant; Rats; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Salmonella typhimurium - genetics; Tumors; Fungi; Ascomycetes; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.37.407

The mutagenicity (Trp+ reversion) of procarcinogens such as N-nitrosodimethylamine, 3, 4-benzpyrene and 2-acetylaminofluorene has been detected with the permeable mutant of yeast, Saccharomyces cerevisiae C658-K42. The original strain C658, however, showed a positive response only to N-nitrosodimethylamine with S9 mix. The permeable mutant C658-K42 was employed for mutagenicity tests on 12 carcinogens which had been reported to be non-mutagenic in Salmonella/microsome tests. It was found that phenobarbital, thiourea, 1, 2-dimethylhydrazine and 4-aminoantipyrine were mutagenic in the presence of S9 mix, Benzene, o-toluidine and thioacetamide were weakly mutagenic. Negative results were obtained for diethylstilbestrol, safrole, acetamide, urethane and ethionine at the concentrations used. Caprolactam did not show any mutagenic effect on C658-K42 at concentrations up to 20 mg/ml. Sodium azide, which is unlikely to be carcinogenic but is strongly mutagenic in the Ames test, showed a very weak mutagenic effect on C658-K42.