Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium

• Published in: Fertility and sterility Vol. 78; no. 4; pp. 787 - 795
• Main Authors: Chung, Hye Won, Lee, Ji Young, Moon, Hye-Sung, Hur, Sung Eun, Park, Mi Hye, Wen, Yan, Polan, Mary Lake
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York, NY Elsevier Inc 01.10.2002; Elsevier Science
• Subjects: Adult; Biological and medical sciences; Blotting, Western; Electrophoresis, Polyacrylamide Gel; endometriosis; Endometriosis - enzymology; Endometriosis - surgery; Endometrium - enzymology; Endometrium - surgery; Female; Female genital diseases; Gene Expression; Gynecology. Andrology. Obstetrics; Humans; Laparoscopy; Matrix Metalloproteinase 2 - analysis; Matrix Metalloproteinase 2 - genetics; Matrix metalloproteinase-2; Matrix Metalloproteinases, Membrane-Associated; Medical sciences; membranous type 1 matrix metalloproteinase; Metalloendopeptidases - analysis; Metalloendopeptidases - genetics; Non tumoral diseases; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; tissue inhibitor of metalloproteinase-2; Tissue Inhibitor of Metalloproteinase-2 - analysis; Tissue Inhibitor of Metalloproteinase-2 - genetics; endometriosis; tissue inhibitor of metalloproteinase-2; membranous type 1 matrix metalloproteinase; Matrix metalloproteinase-2; Human; Enzyme; Enzyme inhibitor; Metalloendopeptidases; Endometriosis; Gene expression; Female genital diseases; Gelatinase A; Peptidases; Uterus; Female genital system; Uterine diseases; Hydrolases; Female; Reverse transcription polymerase chain reaction; Endometrium
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/S0015-0282(02)03322-8

Objective: To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle. Design: Molecular studies in human tissue. Setting: Reproductive immunology laboratory of a university medical center. Patient(s): Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery. Main Outcome Measure(s): Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay. Result(s): Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women. Conclusion(s): Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis.