Metabolome analysis of Escherichia coli ATCC25922 cells treated with high hydrostatic pressure at 400 and 600 MPa

• Published in: Journal of bioscience and bioengineering Vol. 126; no. 5; pp. 611 - 616
• Main Authors: Kimura, Keitarou, Inaoka, Takashi, Yamamoto, Kazutaka
• Format: Journal Article
• Language: English
• Published: Japan Elsevier B.V 01.11.2018
• Subjects: Adaptation, Biological - genetics; Adaptation, Biological - physiology; Capillary electrophoresis–time-of-flight mass spectrometry; Carbohydrate Metabolism - genetics; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli; Glycolytic pathway; High hydrostatic pressure; Hydrostatic Pressure; Metabolic Networks and Pathways - genetics; Metabolome; Metabolome; High hydrostatic pressure; Escherichia coli; Capillary electrophoresis–time-of-flight mass spectrometry; Glycolytic pathway
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2018.05.007

Escherichia coli cells were treated with high hydrostatic pressure (HHP) at 400 and 600 MPa. Metabolites (70–1027 m/z) extracted from HHP-treated cells were analyzed using capillary electrophoresis–time-of-flight mass spectrometry and were compared with those extracted from control cells (not treated with HHP). A total of 133 metabolites were identified and mapped to metabolic pathways, and many of these (42.1%) decreased due to the HHP treatment, including NAD+, NADP+, ATP, and substrates for DNA synthesis. Principal component analysis suggested that the central sugar and nucleic acid metabolic pathways were strongly influenced by HHP. A bottleneck in the central sugar metabolic pathway was observed in HHP-treated cells, which created a metabolic imbalance; metabolites mapped upstream (glucose 6-phosphate, fructose 6-phosphate, and fructose 1,6-diphosphate) were accumulated and those downstream (3-phosphoglycerate, 2-phosphoglycerate, and phosphoenolpyruvate) were depleted. Ribonucleotides were decreased, but the reduction was moderate compared with that of substrates for DNA synthesis; the exception was ATP, which also substantially decreased. The bottleneck in the glycolytic pathway partly explained the exhaustion of ATP. NAD+/NADH ratio of HHP treated cells was comparable with that of untreated control cells.