Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics

• Published in: TheScientificWorld Vol. 2013; no. 2013; pp. 1 - 8
• Main Authors: Galić, Zoran, Orlović, S., Ždero, Ružica, Štajner, D., Popović, B. M.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2013; Hindawi Limited
• Subjects: Antimicrobial agents; Antioxidants; Antioxidants - chemistry; Chemistry Techniques, Analytical; Chemometrics; Correlation analysis; Ethanol; Ethanol - chemistry; Flavonoids; Flavonoids - chemistry; Free Radicals - chemistry; Hydroxyl Radical - chemistry; Leaves; Lipid Peroxidation; Lipids; Multivariate Analysis; Oak; Organs; Peroxidation; Phenol - chemistry; Phenols; Pigmentation; Pigments; Plant Extracts - chemistry; Plant species; Polyphenols; Principal Component Analysis; Principal components analysis; Proanthocyanidins - chemistry; Proline; Proline - chemistry; Quercus - chemistry; Quercus petraea; Quercus robur; Spectrophotometry; Studies; Tannins - chemistry; Trees; Serbia
• ISSN: 2356-6140; 1537-744X
• DOI: 10.1155/2013/134656

Antioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against D P P H • , N O • , and O 2 ∙ - radicals, ferric reducing antioxidant power (FRAP), total phenol, tannin, flavonoid, and proanthocyanidin contents. Permanganate reducing antioxidant capacity (PRAC) was determined using water extracts. Beside, mentioned parameters, soluble proteins, lipid peroxidation (LP), pigments and proline contents were also determined. The data of different procedures were compared and analyzed by multivariate techniques (correlation matrix calculation and principal component analysis (PCA)). PCA found that investigated organs of two different oak tree species possess similar antioxidant characteristics. The superior antioxidant characteristics showed oak leaves over twigs and acorns and seem to be promising source of antioxidants with possible use in industry and pharmacy.