Identification of HUGT1 as a potential BiP activator and a cellular target for improvement of recombinant protein production using a cDNA screening system

• Published in: Molecules and cells Vol. 27; no. 5; pp. 577 - 582
• Main Authors: Yuan Ku, Sebastian Chih (Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore), Lwa, Teng Rhui (Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore), Giam, Maybelline (Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore), Sim Yap, Miranda Gek (Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore), Chao, S.H. (Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore), E-mail: jimmy_chao@bti.a-star.edu.sg
• Format: Journal Article
• Language: English
• Published: Springer Korean Society for Molecular and Cellular Biology 01.05.2009; 한국분자세포생물학회
• Subjects: Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - metabolism; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Biology; Cell Line; CELLS; CELLULE; CELULAS; Endoplasmic Reticulum; Endoplasmic Reticulum Chaperone BiP; Erythropoietin - genetics; Erythropoietin - metabolism; Gene Library; Gene Targeting - methods; Gene Targeting - trends; Genetic Engineering - trends; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; HEK293; HUGT1; Humans; Interferon-gamma - genetics; Interferon-gamma - metabolism; Life Sciences; monoclonal antibody; Monosaccharide Transport Proteins - genetics; Monosaccharide Transport Proteins - metabolism; Protein Folding; recombinant protein production; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Transcriptional Activation; unfolded protein response; 생물학; unfolded protein response; HUGT1; HEK293; monoclonal antibody; recombinant protein production
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-009-0078-z

The development of a high-throughput functional genomic screening provides a novel and expeditious approach in identifying critical genes involved in specific biological processes. Here we describe a cell-based cDNA screening system to identify the transcription activators of BiP, an endoplasmic reticulum (ER) chaperone protein. BiP promoter contains the ER stress element which is commonly present in the genes involved in unfolded protein response (UPR) that regulates protein secretion in cells. Therefore, the positive regulators of BiP may also be utilized to improve the recombinant protein production through modulation of UPR. Four BiP activators, including human UDP-glucose:glycoprotein glucosyltransferase 1 (HUGT1), are identified by the cDNA screening. Overexpression of HUGT1 leads to a significant increase in the production of recombinant erythropoietin, interferon γ, and monoclonal antibody in HEK293 cells. Our results demonstrate that the cDNA screening for BiP activators may be effective to identify the novel BiP regulators and HUGT1 may serve as an ideal target gene for improving the recombinant protein production in mammalian cells.