Mouse Lysozyme M Gene: Isolation, Characterization, and Expression Studies

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 85; no. 17; pp. 6232 - 6236
• Main Authors: Cross, Michael, Mangelsdorf, Inge, Wedel, Angela, Renkawitz, Rainer
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.09.1988; National Acad Sciences
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cell lines; Cloning, Molecular; Complementary DNA; DNA; DNA - genetics; DNA - isolation & purification; Exons; Fundamental and applied biological sciences. Psychology; Genes; Genes. Genome; Isoenzymes - genetics; L cells; Macrophages; Messenger RNA; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Muramidase - genetics; Myeloid cells; Organ Specificity; RNA; RNA, Messenger - genetics; Spleen; Transcription, Genetic; Vertebrata; Mammalia; Gene; Mouse; Nucleotide sequence; Enzyme; Rodentia; Tissue specificity; Gene expression; Lysozyme
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.85.17.6232

We have isolated and characterized both cDNA and genomic DNA of the mouse lysozyme M gene. Derivation of the amino acid sequence from the nucleotide sequences revealed six positions in the carboxyl terminus that differ from partial sequences previously published. The differential detection of specific mRNAs from the closely related lysozyme M and P genes has revealed different but overlapping tissue specificities of expression. The M gene is expressed weakly in myeloblasts, moderately in immature macrophages, and strongly in both mature macrophages and macrophage-rich tissues, while high levels of P transcripts are present only in small intestine. Sites of protein accumulation, rather than gene expression, have been identified by comparative quantitation of mRNA and enzyme levels.