Menstrual endometrial cells from women with endometriosis demonstrate increased adherence to peritoneal cells and increased expression of CD44 splice variants

• Published in: Fertility and sterility Vol. 93; no. 6; pp. 1745 - 1749
• Main Authors: Griffith, Jason S., M.D, Liu, Ya-Guang, M.D, Tekmal, Rajeshwar R., Ph.D, Binkley, Peter A., B.S, Holden, Alan E.C., Ph.D, Schenken, Robert S., M.D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2010
• Subjects: adherence; Base Sequence; CD44; Cell Adhesion - physiology; Cells, Cultured; Endometriosis; Endometriosis - pathology; endometrium; Endometrium - pathology; Endometrium - physiopathology; Epithelium - pathology; Epithelium - physiopathology; Female; Humans; hyaluronan; Hyaluronan Receptors - genetics; Hyaluronan Receptors - metabolism; Internal Medicine; Menstrual Cycle - physiology; Obstetrics and Gynecology; Peritoneal Diseases - pathology; Peritoneum - metabolism; Peritoneum - pathology; Peritoneum - physiology; Protein Isoforms - genetics; Protein Isoforms - metabolism; splice variant; Stromal Cells - metabolism; Stromal Cells - pathology; Stromal Cells - physiology; Up-Regulation - genetics; endometrium; Endometriosis; splice variant; hyaluronan; adherence; CD44
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/j.fertnstert.2008.12.012

Objective We previously demonstrated that adherence of endometrial epithelial (EECs) and stromal cells (ESCs) to peritoneal mesothelial cells (PMCs) is partly regulated by ESC/EEC CD44 interactions with PMC associated hyaluronan. CD44, a transmembrane glycoprotein and major ligand for hyaluronan, has numerous splice variants which may impact hyaluronan binding. Here, we assessed whether ESCs and EECs from women with endometriosis demonstrate increased adherence to PMCs and examined CD44 splice variants' potential role in this process. Design In vitro study. Setting Academic medical center. Patient(s) Fertility patients with and without endometriosis. Intervention(s) Menstrual endometrium was collected from women with and without endometriosis confirmed surgically. The adherence of ESC/EECs to PMCs was measured. The ESC/EEC CD44 splice variants were assessed using dot-blot analysis. Result(s) The ESCs and EECs from women with endometriosis demonstrated increased adherence to PMCs. The predominant CD44 splice variants expressed by ESCs and EECs from women with and without endometriosis were v3, v6, v7, v8, v9, and v10. The ESCs and EECs from women with endometriosis were more likely to express v6, v7, v8, and v9. Conclusion(s) Increased eutopic endometrial-PMC adherence and CD44 splice variant expression may contribute to the histogenesis of endometriotic lesions. Elucidation of factors controlling this expression may lead to novel endometriosis therapies.