Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccination parameters

• Published in: Cancer Immunology, Immunotherapy Vol. 61; no. 11; pp. 2003 - 2011
• Main Authors: Aarntzen, Erik H. J. G., de Vries, I. Jolanda M., Göertz, Joop H., Beldhuis-Valkis, Marjo, Brouwers, Huberdina M. L. M., van de Rakt, Mandy W. M. M., van der Molen, Renate G., Punt, Cornelis J. A., Adema, Gosse J., Tacken, Paul J., Joosten, Irma, Jacobs, Joannes F. M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.11.2012; Springer; Springer Nature B.V
• Subjects: Adjuvants; Adjuvants, Immunologic - therapeutic use; Antibodies - blood; Antigens; Antineoplastic agents; Biological and medical sciences; biomarkers; Bladder cancer; Cancer; Cancer Research; Cancer Vaccines - immunology; Cancer Vaccines - therapeutic use; Clinical trials; Dendritic cells; Dendritic Cells - immunology; Enzyme-Linked Immunosorbent Assay; Female; Haptens; Hemocyanins; Hemocyanins - immunology; Humans; Immune response (humoral); Immunity, Humoral; Immunocompetence; Immunogenicity; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunology; Immunostimulation; Immunotherapy; Male; Medical sciences; Medicine; Medicine & Public Health; Melanoma; Melanoma - immunology; Melanoma - therapy; Metastases; Oncology; Original; Original Article; Pharmacology. Drug treatments; Reproducibility of Results; Retrospective Studies; T cell receptors; Urinary bladder; Vaccination; Vaccines; Netherlands; Dendritic cell; Adjuvant; Immunocompetence; Vaccine; Humoral response; Keyhole limpet hemocyanin; Human; Immune response; Limpet; Hemocyanin; Vaccination; Patient; Gastropoda; Malignant tumor; Cell immunotherapy; Prevention; Treatment; Antigen presenting cell; Parameter; Invertebrata; Mollusca; Humoral immunity; Cancer
• ISSN: 0340-7004; 1432-0851
• DOI: 10.1007/s00262-012-1263-z

Purpose Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it has not been possible to monitor the dynamics of KLH-specific antibody (Ab) responses after in vivo KLH exposure. We designed a quantitative assay to measure KLH-specific Abs in humans and retrospectively studied the relation between vaccination parameters and the vaccine-induced anti-KLH Ab responses. Experimental design Anti-KLH Abs were purified from pooled serum of melanoma patients who have responded to KLH as a vaccine adjuvant. Standard isotype-specific calibration curves were generated to measure KLH-specific Ab responses in individual serum samples using ELISA. Results KLH-specific IgM, IgA, IgG and all IgG-subclasses were accurately measured at concentrations as low as 20 μg/ml. The intra- and inter-assay coefficients of variation of this ELISA were below 6.7 and 9.9 %, respectively. Analyses of 128 patients demonstrated that mature DC induced higher levels of KLH-specific IgG compared to immature DC, prior infusion with anti-CD25 abolished IgG and IgM production and patients with locoregional disease developed more robust IgG responses than advanced metastatic melanoma patients. Conclusions We present the first quantitative assay to measure KLH-specific Abs in human serum, which now enables monitoring both the dynamics and absolute concentrations of humoral immune responses in individuals exposed to KLH. This assay may provide a valuable biomarker for the immunogenicity and clinical effectiveness of KLH-containing vaccines and therapies.