Overexpression of Aspergillus aculeatus cellobiohydrolase I in Aspergillus oryzae

To express the cbhI gene, encoding Aspergillus aculeatus cellobiohydrolase I (CBHI), in Aspergillus oryzae, a plasmid was constructed. The strain that displayed the strongest CBHI activity among the transformants produced about 941 mg/ l in liquid culture. It was confirmed by a PCR method that the p...

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Published inJournal of bioscience and bioengineering Vol. 95; no. 6; pp. 627 - 629
Main Authors Kanamasa, Shin, Mochizuki, Miho, Takada, Goro, Kawaguchi, Takashi, Sumitani, Jun-Ichi, Araii, Motoo
Format Journal Article
LanguageEnglish
Published Japan Elsevier B.V 2003
Elsevier Limited
Subjects
ASPERGILLUS
Aspergillus aculeatus
ASPERGILLUS ORYZAE
avicelase III
cellobiohydrolase I
CELLOBIOSE
GENE EXPRESSION
HYDROLASES
Aspergillus oryzae
cellobiohydrolase I
avicelase III
Aspergillus aculeatus
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Summary:To express the cbhI gene, encoding Aspergillus aculeatus cellobiohydrolase I (CBHI), in Aspergillus oryzae, a plasmid was constructed. The strain that displayed the strongest CBHI activity among the transformants produced about 941 mg/ l in liquid culture. It was confirmed by a PCR method that the plasmid was integrated at the niaD locus.
Bibliography:F60
2004003730
F30
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ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(03)80174-3