Morphological remodeling of the intramural coronary resistance artery network geometry in chronically Angiotensin II infused hypertensive female rats

• Published in: Heliyon Vol. 6; no. 4; p. e03807
• Main Authors: Monori-Kiss, Anna, Antal, Peter, Szekeres, Maria, Varbiro, Szabolcs, Fees, Alexander, Szekacs, Bela, Nadasy, Gyorgy L.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2020; Elsevier
• Subjects: Aging; Angiotensin II; Cardiology; Cardiovascular system; Circulatory system; Coronary arteries; Hypertension; Network; Pathology; Pathophysiology; Physiology; Remodeling; Resistance arteries; Cardiovascular system; Hypertension; Pathophysiology; Remodeling; Pathology; Network; Aging; Resistance arteries; Physiology; Circulatory system; Cardiology; Angiotensin II; Coronary arteries
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2020.e03807

Segmental remodeling of resistance arteries, inhibition of angiogenetic processes, their rarefaction by AngiotensinII and hypertension are accepted facts. Less is known about alterations in resistance artery network geometry potentially induced by them. Female rats were infused with 100 ng/kg/min AngiotensinII with osmotic minipumps for four weeks that raised mean arterial blood pressure from 98 ± 3 to 125 ± 7 mmHg. Geometry of the left coronary artery system was studied on plastic casts and on in situ microsurgically prepared, saline infused video-microscoped networks (n = 13 and 11 controls and hypertensives, respectively). Parallel running branches, broken course of larger branches, multiple branchings and branch crossings have been identified (13 and 74 such deformities, in control and hypertensive networks, respectively, p < 0.01). Bifurcation angles increased with increasing asymmetry of daughter branches but not in hypertensives. Dividing the whole network (theoretically) into several hundreds of 50μm long ring units, ring frequency peaked at 200μm diameter in normal networks. This peak diminished and was replaced by a peak at 300μm in hypertensives (p < 0.01). In controls, diameter of vascular units decreased at a fairly even rate with flow distance from the orifice. The 350, 200, 150μm diameter units were found with highest frequencies at flow distances around 2.5, 5.5 and 7.5mm, respectively. This regular pattern disintegrated in hypertensives. Higher blood flow routes were needed to cover the same distance from the orifice (p < 0.01). Shrinkage and diminishment of many parallel connected 200μm segments, concomitant enlargement of many larger segments accompanied with morphological deformities can be expected to contribute to elevated vascular resistance. Aging; Pathophysiology; Cardiovascular System; Circulatory System; Physiology; Pathology; cardiology; hypertension, resistance arteries, network, remodeling, angiotensin II, coronary arteries.