Stoichiometry of the human glycine receptor revealed by direct subunit counting

The subunit stoichiometry of heteromeric glycine-gated channels determines fundamental properties of these key inhibitory neurotransmitter receptors; however, the ratio of α1- to β-subunits per receptor remains controversial. We used single-molecule imaging and stepwise photobleaching in Xenopus ooc...

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Published inThe Journal of neuroscience Vol. 32; no. 37; pp. 12915 - 12920
Main Authors Durisic, Nela, Godin, Antoine G, Wever, Claudia M, Heyes, Colin D, Lakadamyali, Melike, Dent, Joseph A
Format Journal Article
LanguageEnglish
Published United States Society for Neuroscience 12.09.2012
Subjects
Animals
Brief Communications
Cells, Cultured
Female
Humans
Oocytes - chemistry
Oocytes - metabolism
Protein Subunits - analysis
Receptors, Glycine - chemistry
Receptors, Glycine - classification
Receptors, Glycine - metabolism
Xenopus laevis
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Summary:The subunit stoichiometry of heteromeric glycine-gated channels determines fundamental properties of these key inhibitory neurotransmitter receptors; however, the ratio of α1- to β-subunits per receptor remains controversial. We used single-molecule imaging and stepwise photobleaching in Xenopus oocytes to directly determine the subunit stoichiometry of a glycine receptor to be 3α1:2β. This approach allowed us to determine the receptor stoichiometry in mixed populations consisting of both heteromeric and homomeric channels, additionally revealing the quantitative proportions for the two populations.
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Author contributions: N.D., C.D.H., M.L., and J.A.D. designed research; N.D. and C.M.W. performed research; N.D., A.G.G., and C.M.W. analyzed data; N.D., C.D.H., M.L., and J.A.D. wrote the paper.
M.L. and J.A.D. contributed equally to this work.
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.2050-12.2012