Tap water as a natural vehicle for microorganisms shaping the human gut microbiome

• Published in: Environmental microbiology Vol. 24; no. 9; pp. 3912 - 3923
• Main Authors: Lugli, Gabriele Andrea, Longhi, Giulia, Mancabelli, Leonardo, Alessandri, Giulia, Tarracchini, Chiara, Fontana, Federico, Turroni, Francesca, Milani, Christian, Sinderen, Douwe, Ventura, Marco
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.09.2022; Wiley Subscription Services, Inc
• Subjects: Bacteria - genetics; Biodiversity; Colonization; Dark matter; Drinking Water; Fresh water; Freshwater; Gastrointestinal Microbiome; Genomes; Geographical locations; Homology; Humans; Inland water environment; Intestinal flora; Intestinal microflora; Metagenomics; Microbial activity; Microbiomes; Microbiota; Microorganisms; RNA, Ribosomal, 16S - genetics; Special Issue on Microbiomes and Microbial Communities; Water analysis; Water sampling
• ISSN: 1462-2912; 1462-2920; 1462-2920
• DOI: 10.1111/1462-2920.15988

Summary Fresh potable water is an indispensable drink which humans consume daily in substantial amounts. Nonetheless, very little is known about the composition of the microbial community inhabiting drinking water or its impact on our gut microbiota. In the current study, an exhaustive shotgun metagenomics analysis of the tap water microbiome highlighted the occurrence of a highly genetic biodiversity of the microbial communities residing in fresh water and the existence of a conserved core tap water microbiota largely represented by novel microbial species, representing microbial dark matter. Furthermore, genome reconstruction of this microbial dark matter from water samples unveiled homologous sequences present in the faecal microbiome of humans from various geographical locations. Accordingly, investigation of the faecal microbiota content of a subject that daily consumed tap water for 3 years provides proof for horizontal transmission and colonization of water bacteria in the human gut.