Cloning of heat-shock locus 93D from Drosophila melanogaster

• Published in: The EMBO journal Vol. 3; no. 11; pp. 2499 - 2504
• Main Authors: Waldorf, U, Richter, S, Ryseck, R.P, Steller, H, Edstrom, J.E, Bautz, E.K.F, Hovemann, B
• Format: Journal Article
• Language: English
• Published: England 01.11.1984
• Subjects: Animals; Base Sequence; Cloning, Molecular; DNA Restriction Enzymes; Drosophila melanogaster; Drosophila melanogaster - genetics; Drosophilidae; Genes; genetics; Heat-Shock Proteins - genetics; Molecular Weight; Nucleic Acid Hybridization; Plasmids; Repetitive Sequences, Nucleic Acid; Transcription, Genetic
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1002/j.1460-2075.1984.tb02163.x

Using the microcloning approach a number of recombinant lambda phages carrying DNA from the 93D region have been isolated. Screening genomic libraries, cloned in phage lambda or cosmid vectors, with this isolated DNA yielded a series of overlapping DNA fragments from the region 93D6‐7 as shown by in situ hybridization to polytene chromosomes. In vitro 32P‐labelled nuclear RNA prepared from heat‐shocked third instar larvae hybridized specifically to one fragment within 85 kb of cloned DNA. The region which is specifically transcribed after heat shock could be defined to a cluster of internally‐repetitive DNA and its neighbouring proximal sequences. Over a sequence of 10‐12 kb in length the DNA is cut into repeat units of approximately 280 nucleotides by the restriction endonuclease TaqI. The TaqI repeat sequences are unique in the Drosophila genome.