Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

• Published in: Journal of clinical periodontology Vol. 34; no. 2; pp. 164 - 171
• Main Authors: Kozlovsky, Avital, Artzi, Zvi, Hirshberg, Avraham, Israeli-Tobias, Chen, Reich, Liat
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2007; Blackwell
• Subjects: Amines - therapeutic use; Animals; Anti-Infective Agents, Local - therapeutic use; antimicrobial; Biological and medical sciences; chlorhexidine; Chlorhexidine - analogs & derivatives; Chlorhexidine - therapeutic use; Connective Tissue - drug effects; Connective Tissue - physiology; Connective Tissue Cells - drug effects; Dentistry; Drug Combinations; Epithelial Cells - drug effects; Epithelial Cells - physiology; excisional; Facial bones, jaws, teeth, parodontium: diseases, semeiology; healing; Male; Medical sciences; Mouth Mucosa - cytology; Mouth Mucosa - drug effects; Non tumoral diseases; Otorhinolaryngology. Stomatology; Palate - cytology; Palate - drug effects; Palate - physiology; Palate - surgery; Rats; Rats, Wistar; Regeneration - drug effects; Salicylates - therapeutic use; Terpenes - therapeutic use; Tin Fluorides - therapeutic use; Treatment Outcome; wound; Wound Healing - drug effects; antimicrobial; Rat; Rodentia; Guanidines; Histology; Dentistry; Wound; Antimicrobial agent; excisional; healing; Vertebrata; Biguanides; Mammalia; Animal; Antiseptic; Chlorhexidine; Palate; Disinfecting agent; Morphometry; Cicatrization
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2006.01033.x

Aim: To assess the effect of topically applied antimicrobial agents on palatal excisional wound in rats. Materials and Methods: Excisional wounds, 5 mm in diameter, were made in the centre of the palate of 125 Wistar male rats. In four experimental groups, chlorhexidine digluconate (CHX) 0.12% solution, 1% CHX gel, phenolic compounds solution (Listerine®), amine/stannous fluoride solution (Meridol®) and saline solution as a control group were applied daily for 1 min. The wound area was measured photographically and the epithelialization rate was determined histologically at 3, 7, 14 and 21 days post‐surgery. Results: The mean wound area and mean distance between the epithelial margins decreased significantly with time (p<0.001) in experimental and control groups, with the greatest wound area reduction and rate of epithelialization on day 14. A significantly superior rate of wound epithelialization (p=0.03) was presented following use of 1% CHX gel and Listerine® and a comparatively inferior one when the Meridol® solution was applied. Conclusions: Each tested antimicrobial agent when applied on an excisional wound with epithelial and connective tissue deficiency did not have a negative effect on the rate of wound closure. The best results were achieved with 1%CHX gel and Listerine®.