Polydopamine-functionalized monolithic silica column for mixed-mode chromatography

• Published in: Journal of separation science Vol. 36; no. 9-10; pp. 1516 - 1523
• Main Authors: Zhou, Qiaoli, Yang, Peiling, Xiao, Xing, Jia, Li
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.05.2013; Wiley; Wiley Subscription Services, Inc
• Subjects: Adsorption; Alkylbenzenes; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography - instrumentation; Chromatography - methods; Dopamine; Electrostatics; Exact sciences and technology; Hydrophobic and Hydrophilic Interactions; Indoles - chemistry; Microbiology; Mixed-mode chromatography; Monolithic silica column; Other chromatographic methods; Phenols; Polycyclic aromatic hydrocarbons; Polydopamine; Polymers - chemistry; Resultants; Separation; Silica; Silicon dioxide; Silicon Dioxide - chemistry; Capillary column; Performance evaluation; Purine nucleoside; Purine derivatives; Monolithic silica column; Indole derivative polymer; Chromatographic retention; HPLC chromatography; Polydopamine; Mixed-mode chromatography; Retention time; Surface structure; Persistent organic pollutant; Characterization; Cytosine; Scanning electron microscopy; Adenosine; Hydrocarbon; Monolithic column; Adenine; Polycyclic aromatic compound; Ultraviolet detector; Morphology; Preparation; Phenols; Pyrimidine derivatives; Mixed adsorbent; Uracil
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201201163

Dopamine is easy to self‐polymerize under alkaline conditions and the resultant polydopamine is easy to adhere to the surface of many organic and inorganic materials. Based on the characteristics of dopamine, in this paper, a new polydopamine functionalized monolithic silica column was successfully prepared for performing mixed‐mode chromatography. The performance of the column was evaluated by the separation of different types of samples including alkylbenzenes, polycyclic aromatic hydrocarbons, aromatic acids, phenols, and bases. The mechanism for the separation of these compounds was studied and appeared to involve the mixed interactions containing π‒π, hydrophobic, electrostatic, and hydrophilic interactions.