Urinary peptide profiling identifies a panel of putative biomarkers for diagnosing and staging endometriosis

• Published in: Fertility and sterility Vol. 95; no. 4; pp. 1261 - 1266.e6
• Main Authors: El-Kasti, Muna M., Ph.D, Wright, Cynthia, Ph.D, Fye, Haddy K.S., B.Sc, Roseman, Fenella, M.Sc, Kessler, Benedikt M., Ph.D, Becker, Christian M., M.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 15.03.2011; Elsevier
• Subjects: Adult; Biological and medical sciences; Biomarkers; Biomarkers - urine; desorption; Disease Progression; endometriosis; Endometriosis - diagnosis; Endometriosis - pathology; Endometriosis - urine; Female; Female genital diseases; Gynecology. Andrology. Obstetrics; Humans; Internal Medicine; ionization; laparoscopy; MALDI-TOF; mass spectrometry; matrix-assisted laser desorption-ionization mass spectrometry; Medical sciences; Middle Aged; Non tumoral diseases; noninvasive diagnosis; Obstetrics and Gynecology; pain; patients; Peptides - urine; Pilot Projects; Prospective Studies; Protein Array Analysis - methods; proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; urine; women; Young Adult; noninvasive diagnosis; MALDI-TOF; proteomics; urine; Biomarkers; endometriosis; mass spectrometry; Urine; Peptides; Staging; Gynecology; Stage classification; Biological marker; Exploration; Endometriosis; Matrix assisted laser desorption ionization; Obstetrics; Female genital diseases; Uterine diseases; Diagnosis; Mass spectrometry
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/j.fertnstert.2010.11.066

Objective To identify a potential diagnostic endometriosis marker using matrix-enhanced laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)-based urinary proteomics. Design Prospective randomized pilot study. Setting University hospital, tertiary referral center for endometriosis. Patient(s) 53 women undergoing laparoscopic surgery for pain and/or infertility comprising 30 women without endometriosis and 23 with endometriosis. Intervention(s) Laparoscopy and urine specimens. Main Outcome Measure(s) Urinary peptide profiles. Result(s) We observed distinct patterns of peptide profiles in the urine samples of women presenting with typical clinical symptoms of endometriosis. Six statistically significant putative peptide markers were identified (four during the periovulatory phase and two during the luteal phase) by comparing controls with moderate/severe endometriosis patients. The periovulatory peptide mass of 1,767.1 Da and the luteal peptide mass of 1,824.3 Da both showed a sensitivity of 75% and a specificity of 85% and 71%, respectively. Also detected were seven peptide markers (two during the periovulatory phase and five during the luteal phase) by comparing the urinary peptide profiles of patients with minimal/mild to moderate/severe endometriosis. The periovulatory peptide mass of 3,280.9 Da and the luteal peptide mass of 1,933.8 Da showed a sensitivity of 82% and 75% and a specificity of 88% and 75%, respectively. Conclusion(s) Urinary proteomic analysis may provide a novel method of diagnosing and staging endometriosis.