Oxygen Tension and Riboflavin Gradients Cooperatively Regulate the Migration of Shewanella oneidensis MR-1 Revealed by a Hydrogel-Based Microfluidic Device

is a model bacterial strain for studies of bioelectrochemical systems (BESs). It has two extracellular electron transfer pathways: (1) shuttling electrons an excreted mediator riboflavin; and (2) direct contact between the -type cytochromes at the cell membrane and the electrode. Despite the extensi...

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Published inFrontiers in microbiology Vol. 7; p. 1438
Main Authors Kim, Beum Jun, Chu, Injun, Jusuf, Sebastian, Kuo, Tiffany, TerAvest, Michaela A, Angenent, Largus T, Wu, Mingming
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 20.09.2016
Subjects
Aerotaxis
Bioelectrochemical system (BES)
flavin
Microbiology
Microfluidics
motility
Shewanella
aerotaxis
motility
bioelectrochemical system (BES)
Shewanella
microfluidics
flavin
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Summary:is a model bacterial strain for studies of bioelectrochemical systems (BESs). It has two extracellular electron transfer pathways: (1) shuttling electrons an excreted mediator riboflavin; and (2) direct contact between the -type cytochromes at the cell membrane and the electrode. Despite the extensive use of in BESs such as microbial fuel cells and biosensors, many basic microbiology questions about in the context of BES remain unanswered. Here, we present studies of motility and chemotaxis of under well controlled concentration gradients of two electron acceptors, oxygen and oxidized form of riboflavin (flavin+), using a newly developed microfluidic platform. Experimental results demonstrate that either oxygen or flavin+ is a chemoattractant to The chemotactic tendency of in a flavin+ concentration gradient is significantly enhanced in an anaerobic in contrast to an aerobic condition. Furthermore, either a low oxygen tension or a high flavin+ concentration considerably enhances the speed of This work presents a robust microfluidic platform for generating oxygen and/or flavin+ gradients in an aqueous environment, and demonstrates that two important electron acceptors, oxygen and oxidized riboflavin, cooperatively regulate migration patterns. The microfluidic tools presented as well as the knowledge gained in this work can be used to guide the future design of BESs for efficient electron production.
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Edited by: Jeremy Semrau, University of Michigan, USA
Reviewed by: Sukhwan Yoon, Korea Advanced Institute of Science and Technology (KAIST), South Korea; Jeongdae Im, University of Massachusetts, USA
Present address: Michaela A. TerAvest, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA Largus T. Angenent, Center for Applied Geosciences, University of Tübingen, Tübingen, Germany
These authors have contributed equally to this work.
This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2016.01438