Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings

• Published in: BMC anesthesiology Vol. 18; no. 1; p. 5
• Main Authors: Huang, Wei, Dong, Yunxia, Zhao, Guangyi, Wang, Yuan, Jiang, Jingjing, Zhao, Ping
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 11.01.2018; BioMed Central; BMC
• Subjects: CREB; Dosage and administration; General anesthetics; Health aspects; Isoflurane; Learning; Memory; Offsprings; Patient outcomes; Pregnancy; Psychological aspects; Pregnancy; CREB; Memory; Isoflurane; Offsprings
• ISSN: 1471-2253; 1471-2253
• DOI: 10.1186/s12871-018-0471-2

About 2% of pregnant women receive non-obstetric surgery under general anesthesia each year. During pregnancy, general anesthetics may affect brain development of the fetus. This study aimed to investigate safe dosage range of isoflurane. Forty-eight SpragueDawley (SD) pregnant rats were randomly divided into 3 groups and inhaled 1.3% isoflurane (the Iso1 group), 2.0% isoflurane (the Iso2 group) and 50% O alone (the control group) for 3 h, respectively. Their offsprings were subjected to Morris water maze at day 28 and day 90 after birth to evaluate learning and memory. The expression of cAMP-response element binding protein (CREB) and phosphorylated cAMP-response element binding protein (p-CREB) was detected in the hippocampus dentate gyrus. Less offsprings of Iso2 group were able to cross the platform than that of the control group (P < 0.05). Accordingly, the Iso2 offsprings expressed p-CREB mainly in the subgranular zone in contrast to the whole granular cell layer of hippocampus dentate gyrus as detected in the Iso1 and control offsprings; the expression level of pCREB was also lower in the Iso2 than Iso1 or control offsprings (P < 0.05). Inhalation of isoflurane at 1.3% during pregnancy has no significant influence on learning and memory of the offspring; exposure to isoflurane at 2.0% causes damage to spatial memory associated with inhibition of CREB phosphorylation in the granular cell layer of hippocampus dentate gyrus.