HPLC-DAD Analysis, Antileishmanial, Antiproliferative, and Antibacterial Activities of Lacistema pubescens : An Amazonian Medicinal Plant

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 7
• Main Authors: Mello da Silva, Josiane, Antinarelli, Luciana Maria Ribeiro, Pinto, Nícolas de Castro Campos, Coimbra, Elaine Soares, de Souza-Fagundes, Elaine Maria, Ribeiro, Antônia, Scio, Elita
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; Hindawi Limited
• Subjects: Anti-Bacterial Agents - administration & dosage; Antineoplastic Agents - administration & dosage; Bacteria; Bacterial Physiological Phenomena - drug effects; Biomedical research; Brazil; Cell Proliferation - drug effects; Cell Proliferation - physiology; Cell Survival - drug effects; Chromatography, High Pressure Liquid - methods; Dose-Response Relationship, Drug; Herbal medicine; HL-60 Cells; Humans; Jurkat Cells; Leishmania; Leishmania - drug effects; Leishmania - physiology; Lethal Dose 50; Neoplasms, Experimental - drug therapy; Neoplasms, Experimental - pathology; Parasites; Plant Extracts - administration & dosage; Plants, Medicinal - chemistry; Rheumatism; Studies; Tracheophyta - chemistry; Brazil
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/545038

Species of the genus Lacistema are traditionally used by Brazilian and Peruvian indigenous communities. The present study investigated the in vitro antileishmanial activity against several Leishmania species, cytotoxicity in murine peritoneal macrophages, antiproliferative activity against HL60 and Jurkat cells, and antibacterial activities against seven bacteria strains of the aerial parts of the methanolic crude extract and fractions of Lacistema pubescens. In addition, their chemical profile was also evaluated. Hexane fraction showed the most significant IC50 values against all promastigotes of Leishmania species tested, except for L. chagasi (IC50 = 4.2 µg/mL for L. major and IC50 = 3.5 µg/mL for L. amazonensis). This fraction also exhibited a strong activity against amastigotes of L. amazonensis (IC50 = 6.9 µg/mL). The antiproliferative activity was also observed for methanolic extract and hexane fraction with IC50 = 47.2 µg/mL and IC50 = 39.7 µg/mL for HL60, respectively. Regarding the antimicrobial activity, the overall antibacterial activity was not very significative. Phytol and sitosterol were identified in the methanolic extract. Additionally, previous studies also revealed the presence of those compounds in the hexane fraction. Among other compounds, phytol and sitosterol were probably involved in the antileishmanial and cytotoxicity activities observed in this study.