Drosophila retrotransposon promoter includes an essential sequence at the initiation site and requires a downstream sequence for full activity

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 1; pp. 102 - 104
• Main Authors: Jarrell, K.A. (Harvard University, Cambridge, MA), Meselson, M
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.01.1991; National Acad Sciences
• Subjects: Animals; Base Sequence; Biochemistry; Biological and medical sciences; Developmental genes; DNA; DNA Transposable Elements; DROSOPHILA; Drosophila - genetics; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; Genes; Genic rearrangement. Recombination. Transposable element; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NUCLEOTIDE; NUCLEOTIDOS; Plasmids; Poly A - genetics; Poly A - isolation & purification; Promoter regions; Promoter Regions, Genetic; Retrotransposons; RNA; RNA - genetics; RNA - isolation & purification; RNA Polymerase II - genetics; RNA, Messenger; Terminal repeat sequences; Transcription, Genetic; Transfection; Truncation; Primer extension technique; Nucleotide sequence; Transcription; Transcription promoter; Insecta; Transposable element; Drosophilidae; Arthropoda; LTR sequence; Invertebrata; Drosophila melanogaster; Diptera; Quantitative analysis

We describe a 98-base-pair region (-38 to +60) in the long terminal repeat of the Drosophila gypsy retrotransposon that is sufficient for accurate normal-level transcription. We find that, unlike most RNA polymerase II (pol II) promoters, the gypsy promoter includes downstream sequences that are req...