Drosophila retrotransposon promoter includes an essential sequence at the initiation site and requires a downstream sequence for full activity

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 1; pp. 102 - 104
• Main Authors: Jarrell, K.A. (Harvard University, Cambridge, MA), Meselson, M
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.01.1991; National Acad Sciences
• Subjects: Animals; Base Sequence; Biochemistry; Biological and medical sciences; Developmental genes; DNA; DNA Transposable Elements; DROSOPHILA; Drosophila - genetics; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; Genes; Genic rearrangement. Recombination. Transposable element; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NUCLEOTIDE; NUCLEOTIDOS; Plasmids; Poly A - genetics; Poly A - isolation & purification; Promoter regions; Promoter Regions, Genetic; Retrotransposons; RNA; RNA - genetics; RNA - isolation & purification; RNA Polymerase II - genetics; RNA, Messenger; Terminal repeat sequences; Transcription, Genetic; Transfection; Truncation; Primer extension technique; Nucleotide sequence; Transcription; Transcription promoter; Insecta; Transposable element; Drosophilidae; Arthropoda; LTR sequence; Invertebrata; Drosophila melanogaster; Diptera; Quantitative analysis
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.1.102

We describe a 98-base-pair region (-38 to +60) in the long terminal repeat of the Drosophila gypsy retrotransposon that is sufficient for accurate normal-level transcription. We find that, unlike most RNA polymerase II (pol II) promoters, the gypsy promoter includes downstream sequences that are required for full activity. Also unlike most pol II promoters, the gypsy promoter, which lacks a TATA motif, was found to have an essential sequence at the transcription initiation site, mutation of which abolishes transcription. These three uncommon features of the gypsy promoter may be characteristic of a subset of pol II promoters, exemplified by certain retrotransposons and developmental genes of Drosophila and by Tdt, the mouse terminal deoxynucleotidyl-transferase (TdT) gene.