Kinetic Study of the Intermolecular Interaction between 2-Phenoxypropionic Acid and β-Bromo-cyclodextrin Affixed on the Stationary Phase by Liquid Chromatography

• Published in: Analytical Sciences Vol. 31; no. 8; pp. 743 - 749
• Main Authors: MIYABE, Kanji, KAMIYA, Satoshi
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2015; Springer Nature Singapore
• Subjects: Analytical Chemistry; association rate constant; Chemistry; chiral HPLC system; Chromatography; dissociation rate constant; equilibrium constant; Intermolecular interaction; Langmuir-type rate equation; Ligands; Mathematical analysis; moment analysis; Parking; Rate constants; Reaction kinetics; Strategy; Surface chemistry; Intermolecular interaction; Langmuir-type rate equation; reaction kinetics; dissociation rate constant; chiral HPLC system; moment analysis; equilibrium constant; association rate constant
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.31.743

The intermolecular interaction between 2-phenoxypropionic acid and β-bromo-cyclodextrin affixed on the stationary phase surface in a chiral HPLC system was studied by the moment analysis method. At first, pulse response and peak parking experiments were conducted to measure some parameters concerning the column geometry, adsorption equilibrium, and mass-transfer kinetics. Then, the first absolute moment (μ1) and second central moment (μ2′) of the elution peaks were analyzed by the moment equations, which were developed by assuming that the reaction kinetics between the solute molecules and the functional ligands can be represented by the Langmuir-type rate equation. Finally, the flow-rate dependence of HETP calculated from μ1 and μ2′ was analyzed by using the values of the parameters to determine the association and dissociation rate constants of the intermolecular interaction. It was demonstrated that the combination of the chromatographic experiments and moment analysis is one of the effective strategies for the kinetic study of intermolecular interactions.