Taxonomy and chemical characterization of new antibiotics produced by Saccharothrix SA198 isolated from a Saharan soil

• Published in: Microbiological research Vol. 168; no. 4; pp. 223 - 230
• Main Authors: Boubetra, D., Sabaou, N., Zitouni, A., Bijani, C., Lebrihi, A., Mathieu, F.
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 06.05.2013; Elsevier
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - metabolism; Actinomycetes; Africa, Northern; Anti-Bacterial Agents - metabolism; anti-infective properties; Antibiotics; Antimicrobial activity; Bacteria - drug effects; carbon; Chemical engineering; Chemical Sciences; chemotaxonomy; fungi; Gram-negative bacteria; high performance liquid chromatography; Microbial Sensitivity Tests; minimum inhibitory concentration; Molecular Sequence Data; new products; nuclear magnetic resonance spectroscopy; nucleotide sequences; Phylogeny; radionuclides; ribosomal RNA; Saccharothrix; soil; Soil Microbiology; Taxonomy; toxigenic strains; Algeria; Africa, Northern; Antimicrobial activity; Actinomycetes; Antibiotics; Saccharothrix; Taxonomy
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2012.11.005

Actinomycete strain SA198, isolated from a Saharan soil sample of Algeria, exhibited antimicrobial activity against Gram-positive and Gram-negative bacteria, and phytopathogenic and toxinogenic fungi. The morphological and chemotaxonomic characteristics of the strain were consistent with those of the genus Saccharothrix. Analysis of the 16S rRNA gene sequence of strain SA198 showed a similarity level ranging between 97.2 and 98.8% within Saccharothrix species, S. australiensis being the most closely related. Two new active products were isolated by reverse HPLC using a C18 column. The ultraviolet–visible (UV–VIS), infrared (IR), mass, and 1H and 14C nuclear magnetic resonance (NMR) spectra showed that these products were new bioactive compounds. The minimum inhibitory concentrations of these antibiotics showed a strong activity against fungi and moderate activities against Gram-positive and Gram-negative bacteria.