Outcome of cycles of oocyte in vitro maturation requiring testicular sperm extraction for nonobstructive azoospermia

• Published in: Fertility and sterility Vol. 96; no. 2; pp. 321 - 323
• Main Authors: Fadini, Rubens, M.D, Colpi, Elisabetta, M.D, Mignini Renzini, Mario, M.D, Coticchio, Giovanni, Ph.D, Comi, Ruggero, M.D, Mastrolilli, Massimo, M.D, Guarnieri, Teresa, M.D, Villa, Annalisa, M.D, dal Canto, Mariabeatrice, Ph.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.08.2011; Elsevier
• Subjects: Abortion, Spontaneous - etiology; Adult; animal ovaries; Azoospermia - therapy; Biological and medical sciences; Birth control; Cells, Cultured; compliance; Cryopreservation; Embryo Culture Techniques; Embryo Transfer; Female; fertilization; Gynecology. Andrology. Obstetrics; Humans; in vitro fertilization; infants; Internal Medicine; in vitro maturation; Italy; laws and regulations; Live Birth; Male; Medical sciences; nonobstructive azoospermia; Obstetrics and Gynecology; Oocyte Retrieval - adverse effects; Oocytes; Oocytes - physiology; patients; Pregnancy; Pregnancy Rate; Semen Preservation; Sperm Injections, Intracytoplasmic - adverse effects; Sperm Retrieval - adverse effects; spermatozoa; Sterility. Assisted procreation; Treatment Outcome; women; Italy; in vitro maturation; fertilization; nonobstructive azoospermia; Oocytes; pregnancy rate; Prognosis; Fertilization; Gynecology; In vitro; Obstetrics; Pregnancy; Azoospermia; Testicular sperm extraction; in vitro maturation; Male sterility; Male genital diseases; Oocyte; Oocyte maturation
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/j.fertnstert.2011.05.054

Objective To assess the applicability of oocyte IVM in case of nonobstructive azoospermia (NOA). Design Case series. Setting Private IVF unit. Patient(s) All male partners were affected by NOA. Twenty-one women (20 normo-ovulatory and 1 with polycystic ovary) underwent 27 IVM treatments. The outcome of controlled ovarian stimulation (COS) cycles in normo-ovulatory women whose partners were affected by NOA was assessed for comparison. Intervention(s) Spermatozoa from testicular sperm extraction (TESE) samples were retrieved and cryopreserved before treatment. Main Outcome Measure(s) Rates of fertilization, pregnancy per cycle and transfer, and implantation. Result(s) Twenty-seven IVM-TESE cycles were carried out in 21 women. Fertilization rate was 64.4%. In compliance with national legislation, no more than three oocytes were inseminated in each cycle and all developing embryos were transferred. Six single clinical pregnancies were ascertained (22.2% and 27.3% pregnancy rates per cycle and per transfer, respectively). One pregnancy ended in abortion. The others developed to term and gave rise to five healthy babies. Sixty couples underwent standard COS-TESE treatment. Rates of fertilization, clinical pregnancy per cycle, implantation, and abortion were 64.6%, 20.0%, 11.3%, and 16.7%, respectively. Eleven babies were born from ten pregnancies. Conclusion(s) Oocyte IVM may represent an option in NOA cases.