Hepatocyte-derived Microparticles as Novel Biomarkers for the Diagnosis of Deep Venous Thrombosis in Trauma Patients

• Published in: Clinical and applied thrombosis/hemostasis Vol. 29; p. 10760296231153400
• Main Authors: Zang, Xinwei, Su, Yu, Zhang, Wenjie, Cao, Xiangyu, Li, Chunyan, Lu, Shan, Zhao, Huiru, Chen, Yuying, Liang, Cuiying, Wu, Jun
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.01.2023; SAGE PUBLICATIONS, INC; SAGE Publishing
• Subjects: Biomarkers; Blood Coagulation Tests; Cell-Derived Microparticles; Hepatocytes; Humans; Medical diagnosis; Original Manuscript; Proteins; Thromboembolism; Transmission electron microscopy; Trauma; Venous Thrombosis; trauma; microparticles; diagnosis; deep venous thrombosis; biomarker; flow cytometric analysis
• ISSN: 1076-0296; 1938-2723; 1938-2723
• DOI: 10.1177/10760296231153400

Venous thromboembolism is a common complication following trauma. We investigated the dynamics of plasma microparticles (MPs) levels and explored their potential as biomarkers of deep vein thromboembolism (DVT) after trauma. A total of 775 patients with traumatic fractures were recruited in this nested study. About 106 trauma patients (53 DVT subjects and 53 age-, sex-, and fracture site-matched non-DVT subjects) and 53 healthy volunteers met the enrollment criteria. MPs were characterized by transmission electron microscope, nanoparticle tracking analysis, and western blotting. Circulating levels of MPs were measured using a flow cytometer. Meanwhile, routine laboratory parameters were examined in all patients. Compared to non-DVT patients, DVT patients had higher circulating phosphatidylserine (PS) + MPs, hepatocyte-derived MPs (HMPs), PS + HMPs, and platelet-derived MPs (PMPs). Notably, PS + HMPs had the best predictive value for DVT diagnosis in trauma patients (area under the curve [AUC] 0.8939, 95% CI 0.8326 to 0.9552), which was superior to d-dimer (AUC 0.5881). The Hepatic Procoagulant Index combined plasma levels of PS + HMPs and albumin, increasing the AUC to 0.8978 (95% CI 0.8396 to 0.9561). This is the first study that addressed circulating PS + HMPs are promising biomarkers with high performance in diagnosing DVT. The Hepatic Procoagulant Index is a potential predictor of DVT in trauma patients.