A Diet Including Red Bell Pepper Juice and Soy Protein Suppress Physiological Markers of Muscle Atrophy in Mice

• Published in: Journal of Nutritional Science and Vitaminology Vol. 66; no. 5; pp. 449 - 455
• Main Authors: TACHIBANA, Nobuhiko, FUKAO, Masanori, IRIE, Tomoko, IRISAWA, Yusuke, SHIRONO, Hirotaka, OARADA, Motoko, NIKAWA, Takeshi, FUKAYA, Tetsuya
• Format: Journal Article
• Language: English
• Published: Center for Academic Publications Japan 31.10.2020
• Subjects: metabolome; metabolome analysis; muscle atrophy; red bell pepper; S-adenosylmethionine; soy protein
• ISSN: 0301-4800; 1881-7742
• DOI: 10.3177/jnsv.66.449

Although muscle atrophy can be caused by disuse and lifestyle-related syndromes, it may be possible to prevent this condition through dietary intervention. We hypothesized that a diet including red bell pepper juice (RBPJ) and soy protein isolate (SPI) would prevent muscle atrophy. Accordingly, an experimental diet containing RBPJ and/or SPI was administered for 18 d to normal C57BL/6J mice. The control group was administered a casein diet. Four days before the end of the test period, denervation-induced muscle atrophy and/or sham operation were performed. Anterior tibialis muscle samples were then obtained to assess muscle degradation and perform metabolome analysis. Under the denervation condition, the 20% SPI diet did not alter the mRNA expression levels of muscle atrophy marker genes compared with the 20% casein group. Although the diet comprising RBPJ and 20% casein did not prevent muscle atrophy compared with the control group, the diet containing RBPJ and 20% SPI did. Metabolome analysis revealed that a diet including RBPJ and SPI induced a greater than 1.5-fold change in the levels of 20 muscle atrophy-related metabolites. In particular, the level of S-adenosylmethionine, which concerned with energy metabolism and lifespan, showed a strong positive correlation with the muscle atrophy marker. These findings suggest that a diet including RBPJ and soy protein suppress gene expressions related with muscle atrophy. Further research in humans is needed to confirm whether a combination of RBPJ and SPI can indeed prevent muscle atrophy.