A Recombinant Adenovirus Expressing P12A and 3C Protein of the Type O Foot-and-Mouth Disease Virus Stimulates Systemic and Mucosal Immune Responses in Mice

• Published in: BioMed research international Vol. 2016; no. 2016; pp. 1 - 9
• Main Authors: Xie, Yinli, Li, Zhiyong, Gao, Peng
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: 3C Viral Proteases; Adenoviridae - genetics; Adenovirus; Adenoviruses; Animal diseases; Animals; Antibodies, Viral - blood; Antibodies, Viral - immunology; Antiviral agents; Capsid Proteins - genetics; Capsid Proteins - immunology; Capsid Proteins - metabolism; Cell Proliferation - drug effects; Cysteine Endopeptidases - genetics; Cysteine Endopeptidases - immunology; Cysteine Endopeptidases - metabolism; Cytokines; Cytomegalovirus; Female; Foot & mouth disease; Foot-and-mouth disease; Foot-and-mouth disease virus; Foot-and-Mouth Disease Virus - genetics; Genomes; Grants; HEK293 Cells; Human adenovirus; Humans; Immune response; Immunoglobulin A; Immunoglobulin A - analysis; Immunoglobulin A - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulins; Immunotherapy; Infections; Laboratories; Livestock; Mice; Mice, Inbred BALB C; Plasmids; Proteases; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; Recombinant Proteins - pharmacology; Science; T cells; T-Lymphocytes; Vaccines; Veterinary medicine; Viral proteins; Viral Proteins - genetics; Viral Proteins - immunology; Viral Proteins - metabolism; Viral Vaccines - immunology; Viruses
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2016/7849203

Foot-and-mouth disease (FMD) is a highly contagious livestock disease of cloven-hoofed animals which causes severe economic losses. The replication-deficient, human adenovirus-vectored FMD vaccine has been proven effective against FMD. However, the role of T-cell-mediated antiviral responses and the mucosae-mediated antiviral responses induced by the adenovirus-vectored FMD vaccine was rarely examined. Here, the capsid protein precursor P1-2A and viral protease 3C of the type O FMDV were expressed in replicative-deficient human adenovirus type 5 vector. BALB/c mice immunized intramuscularly and intraperitoneally with recombinant adenovirus rAdv-P12A3C elicited higher FMDV-specific IgG antibodies, IFN-γ, and IL-4 cytokines than those in mice immunized with inactivated FMDV vaccine. Moreover, BALB/c mice immunized with recombinant adenovirus rAdv-P12A3C by oral and intraocular-nasal immunization induced high FMDV-specific IgA antibodies. These results show that the recombinant adenovirus rAdv-P12A3C could resist FMDV comprehensively. This study highlights the potential of rAdv-P12A3C to serve as a type O FMDV vaccine.